Novel Polyoxyethylene-Containing Glycolipids Are Synthesized in Corynebacterium matruchotii and Mycobacterium smegmatis Cultured in the Presence of Tween 80

Table 3

Molecular ions from MALDI mass spectrum of TMS derivative of trehalose derived from purified series-2 glycolipid of C. matruchotii grown in the presence of Tween 80.

MNa^+(m/z)	M	Tentative identification	Relative abundance
726	703	Trehalose(TMS)5	18
798	775	Trehalose(TMS)6	100
870	847	Trehalose(TMS)7	23
942	919	Trehalose(TMS)8	13

Series-2 glycolipid from C. matruchotii was hydrolyzed under basic condition and the aqueous layer in the chloroform/methanol/water two-phase system was desalted. The sugar was converted to their TMS derivative and analyzed by MALDI mass spectrometry.