Novel Polyoxyethylene-Containing Glycolipids Are Synthesized in Corynebacterium matruchotii and Mycobacterium smegmatis Cultured in the Presence of Tween 80

Table 5

Chemical shifts and assignment of proton and ¹³C in NMR of corynomycolate and polyoxyethylenate moieties of purified series-2 glycolipid isolated from C. matruchotii grown in the presence of Tween 80.

	Chemical shift
	Proton (ppm)	^13C (ppm)
C36:2-Corynomycolate^a
Terminal –CH3	0.84	14.0
–CH2–	1.28	29.4
–CH=CH–	5.33	130.5
–C(O) –CH(CH2–)–	2.42	52.4
–C(O) –CH(CH2–)–	1.58	25.4
–CH(OH) –	3.72	73.1
–CH=CH–CH2–	1.96	27.4
–CH2–CH–OH–	1.38, 1.56	34.1
Polyoxyethylenates^b
–CH2–C(O)O–	2.32	34.3
–OCH2–CH2–O–	3.64	70.7
–CH2– (OCH2–CH2–O)m−1^−	5.19	70.2
–CH2– (OCH2–CH2–O)n−1^−	4.60, 4.38	62.9
–OCH2– (ring)	3.71	61.7
–OCH– (ring)	3.63	69.3
–OCH2–CH2–CH2– (ring)	1.93, 2.01	32.8
–OCH–CH2–CH2– (ring)	2.06	33.1

^aStructure of C_36:2-corynomycolate:

^bThe structures of polyoxyethylenates are shown in Figure 3.