C16-Ceramide Analog Combined with Pc 4 Photodynamic Therapy Evokes Enhanced Total Ceramide Accumulation, Promotion of DEVDase Activation in the Absence of Apoptosis, and Augmented Overall Cell Killing
Figure 6
Overall cell killing is promoted after (PDT+LCL30). Following the addition of Pc 4 or LCL30, cells were seeded in the growth medium, preincubated overnight, and irradiated with red light (200 mJ/cm2). After eight days of growth at 37°C, colonies (≥50 cells) were stained with crystal violet (0.1%) and counted. The data are expressed as the percentage of killing and are shown as average ± SEM from six to 26 independent determinations. (a) Dose-dependent increase in cell killing after LCL30. The significance () is shown as follows: * indicates the significant difference between lower doses (0.1 or 1 μM) and the highest LCL 30 dose (5 μM); ~ indicates the significant difference between 0.1 and 1 μM LCL30. (b) Total cell killing is augmented after (PDT + 1 μM LCL30). The significance () is shown as follows: * indicates the significant difference between an individual treatment, LCL30 or PDT, and the combination (PDT+LCL30); ~ indicates the significant difference between LCL30 and PDT.
