Obesity and metabolic syndromes are examples whereby excess energy consumption and energy flux disruptions are causative agents of increased fatness. Because other, as yet elucidated, cellular factors may be involved and because potential treatments of these metabolic problems involve systemic agents that are not adipose depot-specific in their actions, should we be thinking of adipose depot-specific (cellular) treatments for these problems? For sure, whether treating obesity or metabolic syndrome, the characteristics of all adipose depot-specific adipocytes and stromal vascular cells should be considered. The focus of this paper is to begin to align metabolic dysfunctions with specific characteristics of adipocytes.

1. Introduction

Our modern lifestyle has led to the diminished use of consumed energy and to an increasing population of obese individuals. Obesity is costly [1, 2], can result in a poor quality life [2, 3], and causes an early death [1, 2]. Metabolic syndrome is manifested by many symptoms like elevated intramyocellular lipid, intramuscular lipid, blood insulin, glucose, cholesterol, triacylglycerol, increases in blood pressure, risk of cardiovascular disease, and heightened chances of being diagnosed with type 2 diabetes. The progression of both obesity and metabolic syndrome is reaching epidemic proportions [49] and appears to occur at increasingly earlier ages [10]. A general dietary, or metabolic, approach to combat both obesity and metabolic syndrome has had only limited success, but both are generally linked to visceral fat and a host of interacting physiological and pathological processes (Figure 1, Table 1).

2. Adipose Tissue as a Sink for Excessive Fatty Acids

White adipose tissue is critical for the deposition of excessive fatty acids. Surgical implantation of adipose cells into transgenic, adipose-devoid mice reinstated insulin sensitivity, decreased plasma glucose levels, and normalized insulin concentrations [13]. Adipocyte deficiency in human subjects with congenital generalized lipodystrophy is typically accompanied by severe insulin resistance, hyperinsulinemia, hyperglycemia, hypertriglyceridemia, and fatty livers [14]. It has been shown that the efficiency of adipose tissues acting as a repository for excess energy is reduced in transgenic mice which express high levels of preadipose factor-1, resulting in decreased adipose tissue and induction of glucose intolerance and hypertriglyceridemia [15]. Transgenic mice, with 99% less white adipose tissue and inactive brown adipose tissue, developed insulin resistance manifested by threefold elevated plasma glucose levels and 50- to 400-fold elevated insulin levels [16, 17]. White adipose tissue-specific proteins, including perilipin and fat-specific protein of 27 kDa (FSP27), promote sequestration of triglycerides in adipose tissue, which reduces circulatory fatty acid concentration, improving insulin sensitivity in peripheral tissues [18]. FSP27 mediates the formation of unilocular lipid droplet in adipocytes and its knockout enhances lipolysis, leading to insulin resistance [19].

Adipose tissue development is characterized by both adipocyte hyperplasia and hypertrophy. Limiting adipocyte hyperplasia leads to accumulation of lipids in existing adipocytes, resulting in hypertrophy. The proteins downstream of tyrosine kinases-1 (Dok1) are important for adipogenic differentiation and adipocyte hyperplasia. Dok1 activates p120 Ras GTPase-activating proteins, which inhibit the proliferation of preadipocytes and promote their differentiation and lipid accumulation, resulting in hypertrophy of existing adipocytes [20]. It is known that lipid-laden adipocytes are correlated with insulin resistance [21].

In summary, adipose tissue functions as a sink for excessive fatty acids, and the impairment of fatty acid storage function of adipose tissue results in the deposition of fatty acids in other tissues, such as key insulin responsive tissues, muscle and liver, leading to insulin resistance. If metabolic syndromes (specifically) are undiagnosed and allowed to progress, patients will experience persistent hyperinsulinemia, resulting in detectable apoptosis of pancreatic β-cells and Type II diabetes [22].

3. Adipose Depots and Metabolic Syndromes

While visceral adipose tissue appears primarily involved in metabolic syndromes, there are three other major adipose depots that also may have a role in the etiology of obesity and/or metabolic syndrome, including visceral, subcutaneous, intermuscular, and intramuscular (including intramyocellular lipid) depots. Not all adipose depots possess the same physiology, and the distribution of adipocytes within the four major adipose depots plays a role in the severity of obesity and ultimately metabolic syndromes. This is probably due to the inherently different responses by the collective populations of different types of adipocytes in the distinct adipose depots.

Obtaining knowledge of how cell populations in depot-specific adipose tissue manage the dynamics of energy partitioning is of growing importance [23, 24]. However, this is a difficult area of physiology to grasp as not only are the adipose depots different, but so are the adipocytes within any one adipose depot [12]. Moreover, because stromal vascular cells, preadipocytes, and mature adipocytes are sources of proliferative-competent progeny cells in adipose tissue, the traditional descriptions of adipogenesis and differentiation no longer hold true [12]. This implies that novel experimental designs must be employed in order to address depot-specific adipocyte cellularity during development, growth, and metabolism. To confound matters further, the adipose tissue stromal vascular content is also depot dependent and may, in part, dictate the potential of disease severity. Firstly, these cells may provide preadipocytes for increasing the number of lipid-assimilation cells in the depot. Also, as the extent of vascularity and connective tissue composition and deposition may be under intrinsic control different from the adipocyte itself, stromal vascular cells may become a key player in adipocyte physiology. For example, connective tissue deposition and organization are decreased in depots with a higher proportion of larger adipocytes (internal or visceral depots). Impaired adipose tissue blood flow associated with severe adipocyte hypertrophy in these depots may induce hypoxic conditions which, in turn, may destabilize the adipocyte extracellular matrix (ECM) resulting in a number of adverse conditions. Also, severe adipocyte hypertrophy per se may adversely influence both the capillary and adipocyte ECM stability. All components of adipose tissue depots must be considered as being potentially involved in adipose tissue-related disease.

Increasing energy utilization via exercise or weight loss provides a transient opportunity for energy storage in existing adipose cells, improves insulin sensitivity, and allows consumed, but as yet unused glucose, to be stored as lipid. However, if exercise/weight loss is inefficient, what becomes of the unutilized glucose? Moreover, is the manifestation of type II diabetes or hypercholesterolemia an indication of where feedback inhibition of depot-specific cellular metabolic processes exists? Also, is the hypercholesterolemia seen in metabolic syndrome caused by the inability of the body to synthesize lipid from acetate? Moreover, is the manifestation of type II diabetes or hypercholesterolemia an indication of where feedback inhibition of depot-specific cellular metabolic processes exists? Careful scrutiny of adipocytes may well address these questions and will provide knowledge about specific populations of adipocytes in the development of obesity/metabolic syndromes.

4. Targets with Which to Combat Obesity and Metabolic Syndromes

Figure 2 depicts five targets for manipulating adipocytes in order to regulate obesity or metabolic syndrome. Traditional research and clinical focus has been directed towards (1) and (2), the formation of lipid assimilating adipocytes from adipocyte precursors (preadipocytes). Moreover, as the stromal vascular cell fraction of any adipose depot may provide cells of the adipocyte lineage (preadipocytes) a majority of research has traditionally been placed on this cell population. Recent interest has been expanding to include mechanisms in which adipocytes play an active regulatory role in metabolism (3). To this end, data from recent studies suggest that fetal programming of mesodermal cells may play an important role in the accumulation of postnatal adipocytes [25]. Physiological relevant processes as simple as altering the diet of mothers might regulate adipocyte numbers in offspring. Alterations in adipocyte numbers have also been shown via diet manipulations at discernable time points postnatally (4). To be sure, the nutritional plane of mothers during pregnancy results in lower birth weights of babies so the ability to shift the overall cellular make-up during development is not absurd. Moreover, mature adipocyte numbers may not be as fixed as once thought. Adipocytes might be able to dedifferentiate to form additional proliferative-competent progeny cells (5) which might add adipocytes to specific adipose depots, thereby increasing the lipid load [12].

Collectively, knowledge gained in any of these areas will aid us in finding a possible regulatory point for combating energy flux-related dysfunctions. Similarly, the observations contained in Table 1 offer insight into physiological points/observations whereby research might provide knowledge regarding alleviation of adverse symptoms of both obesity and metabolic syndrome. As one example, since adipocytes are capable of synthesizing and releasing chemical regulators into the blood that might affect systemic metabolism/physiology, the identification of the release/effect mechanism might then point to a target for regulation (Figures 1 and 2). In light of this, a systemic remediation regimen might be developed, reducing the effects of adipocyte secretions.

5. Conclusion and Perspectives

Can adipose tissue become the major regulator of body composition? Why is it that one of the most benign/banal tissues, adipose tissue, has become an amazing source of stem cells and systemic regulatory agents and, therefore, controls of a variety of systemic and local physiological and pathological variables of growth, development, and metabolism? In the light of current research, this once thought innocuous tissue has emerged as a true organ system, and these are issues that have to be addressed in the near future to gain more understanding regarding obesity, metabolic syndromes, and adipocytes.

Conflict of Interests

The author declares that there is no conflict of interests.