Research Article

Paraoxonases-2 and -3 Are Important Defense Enzymes against Pseudomonas aeruginosa Virulence Factors due to Their Anti-Oxidative and Anti-Inflammatory Properties

Figure 6

Both PON2 and PON3 are degraded after 3OC12 treatment. (a) A549 cells were treated with 3OC12 (100 μM) for the indicated durations or (b) with thapsigargin (24 h) with the indicated concentrations. Lysates (50 μg of protein) were analyzed by Western blotting using anti-PON2, anti-PON3, or anti-α-tubulin antibodies. One representative blot is shown. Results (right) are the means ± S.E.M. of three replicate analyses; 𝑃 < 0 . 0 1 ; 𝑃 < 0 . 0 0 1 .
352857.fig.006a
(a)
352857.fig.006b
(b)