Research Article

Fatty Acid Composition of Phospholipids and in the Central and External Positions of Triacylglycerol in Muscle and Subcutaneous Fat of Beef Steers Fed Diets Supplemented with Oil Containing n6 and n3 Fatty Acids While Undergoing One of Three 48 h Feed Withdrawal Treatments

Table 2

Unsaturated fatty acid composition of phospholipid (PL), triacylglycerol (TAG), and at sn-2 monoglycerol (sn2) and sn1/3 of fat from the pars costalis diaphragmatis (PCD) of beef steers fed dietary oil composed of an equal mixture of flax oil and sunflower oil at 5% of diet and in one of no feed withdrawal (No FW), single feed withdrawal (FW × 1), and feed withdrawal every 8 wk (FW × 4) for 48 h treatments.

ItemLocationControlOilProbability
No FWNo FWDietFW

Fat (%)1 0.090 0.930 0.778

Fatty acid (wt %)2

16:1c9PL
TAG
sn2
sn1/3


















0.0042
0.0390
0.1470
0.0133
0.5575
0.6145
0.3476
0.7624
0.1625
0.9587
0.9007
0.8757

18:1t9PL
TAG
sn2
sn1/3


















0.0001
0.0001
0.1063
0.0001
0.6047
0.6329
0.3700
0.2974
0.4898
0.5855
0.7840
0.7653

18:1t11PL
TAG
sn2
sn1/3


















0.0001
0.0001
0.1054
0.0001
0.6818
0.1750
0.1810
0.4750
0.3064
0.3088
0.8312
0.5305

18:1c9PL
TAG
sn2
sn1/3


















0.0117
0.0606
0.7381
0.0644
0.3050
0.4334
0.5156
0.6027
0.0557
0.0689
0.5819
0.0493

18:2t9c11PL
TAG
sn2
sn1/3


















0.0001
0.0001

0.0001
0.4224
0.1367

0.1362
0.1577
0.3201

0.3241

18:26c9, 12PL
TAG
sn2
sn1/3


















0.0103
0.0262
0.4018
0.0406
0.4857
0.3416
0.5291
0.3348
0.9137
0.7200
0.7168
0.8670

18:33C9, 12, 15PL
TAG
sn2
sn1/3


















0.0326
0.0001
0.4591
0.0050
0.6321
0.1043
0.2189
0.3475
0.3615
0.1182
0.1301
0.3903

CLAc9t11PL
TAG
sn2
sn1/3


















0.0034
0.0001
0.6811
0.0177
0.5983
0.0665
0.4191
0.1903
0.9584
0.0022
0.4420
0.1687

CLAt10c12PL
TAG
sn2
sn1/3


















6
0.2976
0.3563
0.8140
0.7797
0.3666
0.1712
0.2088
0.2140
0.5446
0.3232
0.6164
0.6239

eported in He et al. [12] fat content is on as is basis.
as in Paterson et al. [10], .