PON1 solubilizing mutations. (a) The surface of G2E6 is shown, with hydrophobic amino acids (VGMCILYFW) shown in red. Residues 1–15 are not resolved in the X-ray crystal structure, but the ΔN-huPON1 variant removed residues 4–17. (b) The positions modified in the ΔHDL-huPON1 variant are shown in spheres. These residues compose much of the hydrophobic surface patch near the N-terminus evident in (a). The Ca2+ ions are shown as pink spheres, and a phosphate bound in the presumed active site is shown in orange sticks. (c) The 59 positions that differ between huPON1 and G2E6 are shown; the positions that were modified in g2e6p-huPON1 are spheres, and the other 43 positions are sticks. Position 166, which was modified because of its proximity of 192, is noted. Rendered from PDB ID: 1V04 with PyMOL.
