The major polar lipids of M. hyorhinis. Lipids were extracted from membrane preparations by the method of Bligh and Dyer [18]. The lipids were separated by TLC on silica gel plates (Kiesel-gel 60 HR, Merck, Darmstadt, Germany) developed at room temperature by a two-dimensional system using chloroform-methanol-ammonia (65 : 35 : 4 by vol.) for the first dimension and chloroform-methanol-acetic acid-water (85 : 25 : 5 : 4 by vol.) for the second dimension. Lipid spots were detected by iodine vapor, while phospholipid spots were detected by the molybdate spray reagent [29]. The spots were tentatively identified based on their comigration with commercial standards as NL, neutral lipids; CL, cardiolipin; PG, phosphatidylglycerol; PC, phosphatidylcholine; SPM, sphingomyelin.
