The Impairment of Macrophage-to-Feces Reverse Cholesterol Transport during Inflammation Does Not Depend on Serum Amyloid A
Figure 2
SAA does not contribute to reduced macrophage to plasma RCT during an acute phase response. WT and SAAKO mice were injected subcutaneously with PBS or 0.8 μg/g LPS as indicated, followed by an intraperitoneal injection of 3H-cholesterol-labeled J774 macrophages 4 hr later. Radioactivity in plasma was determined 4 hr (a) and 24 hr (b) after macrophage injection (8 hr and 28 hr after LPS injection). The data shown in (a) and (b) were compiled from 3 separate experiments (n = 4-5 mice per group per experiment) after normalization to the PBS-injected WT mice. For the 3 experiments, radioactivity recovered in plasma of control WT mice at 4 and 24 hr ranged from 1.0% to 2.4% and 2.0% to 5.0% of the injected radioactivity, respectively. (c) Cholesterol efflux assays were carried out using J774 cells as described in Materials and Methods using serum (2.5% v : v) collected 8 hr after administration of LPS (0.8 μg/g body weight; ). Values are the mean ± SEM; *, ***.
