Trans-10, cis 12-Conjugated Linoleic Acid-Induced Milk Fat Depression Is Associated with Inhibition of PPAR𝜸 Signaling and Inflammation in Murine Mammary Tissue

Table 2

Effect of trans-10, cis-12-CLA diet on milk production, milk lipid content, milk fatty acid profile, liver weight, and liver fatty acid profile.

Item	Treatment			𝑃 value
	Control	CLA	SEM
Milk fat (weight %)	22.55	13.28	0.96	<0.01
Milk production (g)	3.51	2.12	0.53	NS^1
Liver weight (g)	1.85	2.44	0.28	NS
Liver FAME^2 (g)	0.26	0.47	0.03	<0.01
Milk fat (g/100 g FAME)
<16:0	24.18	14.26	1.58	<0.01
16:0	22.42	17.99	0.62	<0.01
>16:0	50.48	66.68	1.67	<0.01
MUFA^3	40.26	48.86	1.54	<0.01
trans-10, cis-12-CLA	ND^4	1.42	0.05	NS
20:4n-6	0.54	1.32	0.09	<0.01
Liver (g/100 g FAME)
16:0	12.88	21.51	0.68	<0.01
18:0	6.54	3.67	0.37	<0.01
18:1 cis-9	55.15	54.01	1.74	NS
trans-10, cis-12-CLA	ND	0.20	0.01	<0.01
18:3	1.51	1.13	0.07	<0.05
20:4n-6	5.11	1.91	0.42	<0.01

 ¹Not significant.
²Fatty acid methyl esters.
³Monounsaturated fatty acids.
⁴Not detected.