Trans-10, cis 12-Conjugated Linoleic Acid-Induced Milk Fat Depression Is Associated with Inhibition of PPAR𝜸 Signaling and Inflammation in Murine Mammary Tissue

Table 5

Verification of selected genes in mammary tissue with RT-qPCR.

Gene	Description	CLA versus control
		Microarray	RT-qPCR	𝑃 value^1
Bax	BCL2-associated X protein	1.11	−1.08	>0.10
Stat5b	Signal transducer and activator of transcription 5B	−1.39	−2.00	<0.01
Rela	v-rel reticuloendotheliosis viral oncogene homolog A	−1.51	−1.10	0.50
Kras	v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog	−1.28	−1.24	0.05
B2m	Beta-2 microglobulin	2.61	−1.57	<0.01
Saa2	Serum amyloid A 2	2.58	5.47	<0.01
Orm1	Orosomucoid 1	3.67	2.66	<0.01
Cp	Ceruloplasmin	2.94	1.09	>0.10
Insig1	Insulin induced gene 1	−1.25	−1.11	>0.10
Defb1	Defensin beta 1	4.56	2.31	<0.01
C4b	Complement component 4B (Childo blood group)	2.09	2.64	<0.01
Pparγ	Peroxisome proliferator activated receptor gamma	−1.34	−2.77	<0.01
Mup2	Major urinary protein 2	−4.58	−11.64	<0.01
Tnf	Tumor necrosis factor	1.24	−1.50	0.09
Saa1	Serum amyloid A 1	2.35	3.84	<0.01
Orm2	Orosomucoid 2	2.28	3.81	<0.01
Srebf1	Sterol regulatory element binding factor 1	−1.40	−1.23	0.01
Scap	SREBF chaperone	1.07	−1.05	>0.10
Gpam	Glycerol-3-phosphate acyltransferase, mitochondrial	−1.08	−1.39	0.01
Scd	Stearoyl-coenzyme A desaturase 1	−3.11	−3.78	<0.01
Fasn	Fatty acid synthase	−2.11	−2.31	<0.01

 ¹RT-qPCR data.