Effects of Myeloperoxidase-Induced Oxidation on Antiatherogenic Functions of High-Density Lipoprotein

Figure 1

Apolipoprotein profiles of HDL treated with and without MPO. HDL treated with (+) or without (−) myeloperoxidase (MPO) for 1 and 24 hrs was subjected to SDS-PAGE followed by staining with Coomassie Brilliant Blue (a) and by immunoblotting for apoA-I and apoA-II (b). The 37 kDa bands, defined as apoA-I/apoA-II heterodimer, on CBB stained gel were indicated by white arrows. The bands that reacted with both anti-apoA-I and anti-apoA-II antibodies were identified as apoA-I/apoA-II heterodimers (black arrow). The molecular masses of standards are listed on the left. The ratios of apoA-I in apoA-I/apoA-II heterodimer to total apoA-I and apoA-II in apoA-I/apoA-II heterodimer to total apoA-II were estimated from immunoblotting patterns using a CS Analyzer (c). , ^†.

(a)

(b)

(c)