High-Density Lipoprotein Binds to Mycobacterium avium and Affects the Infection of THP-1 Macrophages

Figure 1

Interaction between bacteria and HDL. (a) The bacterial samples (lane 1: Pseudomonas aeruginosa, lane 2: Aeromonas species, lane 3: Klebsiella oxytoca, lane 4: Klebsiella pneumoniae, lane 5: Serratia marcescens, lane 6: Citrobacter koseri, lane 7: Pseudomonas aeruginosa, lane 8: Enterobacter cloacae, lane 9: Acinetobacter baumannii, lane 10: Stenotrophomonas maltophilia, lane 11: Mycobacterium avium, and lane 12: Escherichia coli) were mixed with normal human serum (NHS). The NHS conjugated bacteria were washed and solubilized by means of lysis buffer. The lysates were separated by 12.5% SDS-PAGE and immunoblotted with anti-apoA-I antibody. The molecular weight markers are also shown (M). (b) M. avium was incubated with NHS, heat-inactivated serum (HIS), HDL, and apoA-I for 10 min at 37°C. After washing with PBS, M. avium was solubilized and subjected to immunoblotting for apoA-I as described above.

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