Cholesterol Efflux Capacity of Apolipoprotein A-I Varies with the Extent of Differentiation and Foam Cell Formation of THP-1 Cells

Figure 4

Foam cell formation. (a) After PMA treatment for 2 days, cells were loaded with acLDL (50 μg protein/mL) and T0901317 (1 μmol/L) for indicated periods. After equilibration, cells were stained with oil red O and hematoxylin (scale bars, 20 μm). (b) Semiquantitative analysis of oil red O positive cells was performed using the ImageJ (National Institutes of Health). The values were indicated by mean + SD ( = 3, < 0.05 versus day 1, < 0.05 versus day 2, < 0.05 versus day 3, and < 0.05 versus day 4).

(a)

(b)