A Fast and Simple Qualitative Method for Screening Oleaginous Yeasts on Agar

Table 1

Lipid dyes tested for the screening of oleaginous yeasts.

Dye	Solvent	Stock solution concentration; (g/L)	Dye concentration in media	Price; (USD/L culture)	References
Oil Red O	Isopropanol	30	9 mL/mL	230	[9]
Sudan Black B	Ethanol	30	2.4 g/L	7	[8]
Nile Red	Acetone	0.1	10 mg/L	0.5	[6]
Rhodamine B	Ethanol	1	10 mg/L	0.004	[16], this work
Rhodamine B	Ethanol	1	5 mg/L	0.002	This work
Rhodamine B	Ethanol	1	20 mg/L	0.008	This work

Calculated from prices in Sigma-Aldrich (http://www.sigmaaldrich.com/).