Journal of Lipids

Review Article

Realm of Thermoalkaline Lipases in Bioprocess Commodities

Table 1

Characteristics of TA lipases.


TA lipases	MW	(°C)	pH	Stimulants	Inhibitors	Chemicals with tolerance	References

Pseudomonas sp. lipase	-	90	11	-	-	-	[21]
BSK-L lipase	-	30–60	8	Mn²⁺, K⁺, Zn²⁺, Fe²⁺, Ca²⁺	-	SURF, oxidants, detergents	[22]
Microbacterium sp. lipase	40.0	50	8	Tri-X-100, SDS	PMSF, EDTA, DTT, Tw-20, Tw-80	-	[23]
PS3 lipase	31.4	55	7	Tri-X-100, Mg²⁺ Ca²⁺	M+ (A), SDS, EDTA, CTAB, Tw-80, glycerol	Solvents (A)	[24]
S. aureus lipase	-	60	12	-	-	-	[25]
S. pasteurii lipase	56.0	50	9.0	-	-	-	[8]
B. stearothermophilus lipase	67.0	55	9–13	Mg²⁺	-	SURF, oxidants, nonpolar solvent	[26]
BCRC 35396 lipase	60.0	25–60	7–10	-	-	-	[11]
Acinetobacter AU07 lipase	45.0	50	8	-	PMSF, SDS, H₂O₂, Tw-80, TriX-100, EDTA, DTT	-	[27]
LipB lipase	57.0	96	8	Mn²⁺ Ca²⁺	PMSF, EDTA	-	[15]
LipA lipase	50.0	96	8	Mn²⁺ Ca²⁺	PMSF, EDTA	-	[15]
Lip 256 lipase	33.0	80	9	Na⁺, Fe³⁺, K⁺, Fe²⁺, Sr²⁺	Ca²⁺, Mg²⁺, Cu²⁺, Solvent (D)	Glycerol, MaCN, pyridine, urea	[28]
T. thermophilus lipase	39.0	40–70	9.5	Ca²⁺	Zn²⁺, Cu²⁺	-	[29]
RSJ-1 lipase	37.0	50	8-9	Ca²⁺, Na⁺, Mg²⁺, Ba²⁺	Cs⁺, K⁺, Co²⁺, Zn²	EDTA	[30]
ID-1 lipase	34.0	70–75	7.5	Ca²⁺, Zn²⁺	-	-	[31]
LBN2 lipase	33.0	60	10	-	-	-	[9]
Cohnella sp. A01 lipase	29.5	70	8.5	-	-	EDTA	[18]
4R lipase	21.9	80	9.0	Mg²⁺	M+ (B), PMSF, orlistat, OA, I^-, EDTA, urea	-	[4]
Ht7 lipase	22.0	90	9	Ca²⁺ Co²⁺ and Zn²⁺	-	-	[32]
T. atroviride 676 lipase	-	65	3–8	-	-	Solvents (B)	[33]
AV-5 lipase	50.0	65	9	-	-	-	[34]
BTS-3 lipase	31.0	55–70	8–10.5	K⁺, Fe³⁺, Hg²⁺, Mg²⁺	Al³⁺, Co²⁺, Mn^2+, Zn²⁺	-	[35]
CCR11 lipase	11.0	60	9-10	Ca²⁺, Tri-X-100	PMSF, SDS, Tw-80, Tw-20, butanol	-	[36]
T. lanuginosus lipase ln1	33.0	60–70	8–12	Ca²⁺	Fe²⁺, Zn²⁺, K⁺, Ag⁺	-	[37]
B. thermocatenulatus lipase	16.0	60–70	7.5–8	-	-	-	[38]
HF544325 lipase	27.0	45	8	-	-	-	[39]
S. arlettae JPBW-1 lipase	45.0	30–90	7–12	Mn²⁺, Ca²⁺ and Hg²⁺	K⁺, Co²⁺, Fe²⁺	Solvents (C), Tw-80, Tw-40, EDTA	[16, 40]
Bacillus sp. A30-1 lipase	65.0	-	-	-	-	-	[41]
Staphylococcus aureus	25.0	52	11	Ca²⁺ and Tween-80	SDS	-	[42]
Lipase L2	43.0	70	9	Ca²⁺, K⁺, Na⁺, Mn²⁺	EDTA, PMSF, pepstatin A, BME, DTT	-	[17, 43]

Note. MW, molecular weight; T, optimal or favourable temperature of enzyme activity; OA, oleic acid; Tw-20, Tween-20; Tw-80, Tween-80; SURF, surfactants; BME, 2-mercaptoethanol; solvent (A): methanol, ethanol, acetone benzene chloroform xylene; solvent (B): benzene, xylene, n-hexane, methanol, ethanol, and toluene up to 30%; solvent (C): kerosene, n-dodecane, and hexane; solvent (D): acetone, MeOH, trichloromethane, petroleum ether, hexane, isopropanol, DTT, EDTA, polyhexamethylene biguanide, DMSO, benzene, Tri-X-100, Tw-20, Tw-80, SDS; M+ (A): Cu²⁺, Fe²⁺, Zn²⁺, and Co²⁺; M+ (B): Co²⁺, Cd²⁺, Hg²⁺, Cu²⁺, and Pb²⁺; I^-, iodine.