Realm of Thermoalkaline Lipases in Bioprocess Commodities

Table 5

The production of TA lipases via improved strains.

Gene of origin	Expression of lipase gene	Medium	,	Agitation (rpm)	pH	DOT (%), aeration rate (L/min)	Induction	Duration (h)	Activity	References
Enterobacter sp. Bn12	E. coli (BL21) pLysS and pET-26(+)	LB + antibiotics	37, 20	-	-	-, -	0.5 mM IPTG (OD600: 0.6)	18	2900 (U/mg)	[61]
Thermomyces lanuginosus (lipase gene ln1)	Pichia GS115 and expression plasmid vector pPIC9K (Heterologous expression under AOX1 promoter)	BMGY medium	28, 28	220	-	-, -	1.0% (v/v) MeOH daily (OD600: 2.0–4.0)	168	1328 (U/mL)	[66]
Geobacillus thermocatenulatus	E. coli BL321 carrying the PCYTEXP1 plasmid	Modified LB	30, 45	200 to 600	7.0	30, 3	Using temperature-inducible kPL promoter (OD600: 0.4–0.6)	12	770,000 (U/g DCW)	[67]

Note. , temperature for growth; , temperature for production; BMGY, buffered methanol complex medium; AOX1, alcohol oxidase 1 promoter; LB, Luria-Bertani medium; NaCl, sodium chloride; MeOH, methanol; modified LB medium containing 5 g/L NaCl, 10 g/L Bacto yeast extract, 10 g/L Bacto tryptone, 10 g/L glucose, 10 mM MgSO4, and 40 mM K2HPO4, together with 100 lg/mL of ampicillin; DCW, dry cell weight.