Realm of Thermoalkaline Lipases in Bioprocess Commodities

Table 6

The production of TA lipases in bioreactors.

Bioreactors; (size)	Strain	IS (%)	Medium composition (%, v/v or w/v)	Temperature (°C), agitation (rpm)	Aeration rate (L/min), DOT (%)	pH	Other minerals/parameters	Duration (h)	Activity (U/mL)	References
3 L	Acinetobacter sp. AU07	0.5	Castor oil, 2	30, 150	-, -	7	-	12	48	[27]
2 L	G. thermodenitrificans	-	Waste cooking oil, 2	50, 400	1-2, -	8	-	-	330	[34]
2 L	Antrodia cinnamomea BCRC 35396	10	Glucose, 3; peptone, 0.5; yeast extract, 0.3; malt extract, 0.3	28, 150	2, -	4	KH2PO4, 0.1; olive oil, 0.01; vitamin B1, 0.1; MgSO4·7H2O, 0.1	18	26	[11]
20 L	Bacillus sp. RSJ-1	0.1	Peptone, 0.75; yeast extract, 0.75	50, 350	10, -	9.0	NaCl, 0.5; cotton seed oil, 0.75; Tween-80, 0.5; CaCl2·2H2O, 0.001	10	2.13 (U/mg)	[30]
1 L	Citrobacter freundii IIT-BT L139		Starch, 1 and peptone-urea, 1	40, -	-, -	9.0	-	60	12	[12]
14 L	B. cepacia	2	Glucose, 1; NH4Cl, 0.5; (NH4)2HPO4, 0.24	45, 300	4, 25	7	KH2PO4, 0.1; MgSO4·7H2O, 0.01; palm oil emulsified with gum acacia, <1	20	120 (U/mg)	[10]
20 and 100 L	Thermosyntropha DSM	-	Yeast extract, 0.75; NH4Cl, <1; cysteine, <1	60, -	-	8.2	NaCl, K2HPO4, Na2S·9H2O, Na2CO3, MgCl2·6H2O, KCl NaHCO3, CaCl2·2H2O (<1)	15–21	0.12–0.15 (U/mg)	[15, 39]

Note. STB, stirred tank bioreactor; IS, inoculums size; vvm, vessel volumes per minute; DOT, dissolved oxygen tension; NaCl, sodium chloride; K₂HPO₄, potassium hydrogen phosphate; KCl, potassium chloride; Na₂S·9H₂O, sodium sulphide nonahydrate; NH₄Cl, ammonium chloride; MgCl₂·6H₂O, magnesium chloride hexahydrate; CaCl₂·2H₂O, calcium chloride dehydrate; NaHCO₃, sodium bicarbonate; Na₂CO₃, sodium carbonate; C₃H₇NO₂S, cysteine; (NH₄)₃PO₄, ammonium phosphate; BE, beef extract; ECO, emulsified coconut oil.