Figure 1: Treatment with high density lipoprotein (HDL) reduces scavenger receptor class B type 1 (SR-B1) expression in HepG2 cells. (a) Treatment with 15, 62, 125, 250, and 500 ug/ml HDL all significantly reduced protein levels of SR-B1. (b) Representative Western blot showing reduction of SR-B1 levels. Cells were treated with 15–500 ug/ml HDL for 24 hours. Cell lysates were collected and run on 4-12% Bis-Tris gels, transferred to nitrocellulose, probed with anti-SRB1 (1:750) and anti-β-Actin (1:2000) antibodies, and developed using enzyme linked chemiluminescence. Protein levels were quantitated using ImageJ software ( p< 0.01, p<0.05; 15-125 ug/ml, n=4 and 250-500 ug/ml, n=2).