A Transcriptional Regulatory System of the S. cerevisiae OLE1 Gene Responds to Fatty Acid Species and Intracellular Amount, and not Simply Membrane Status

Figure 1

Fatty acid composition of cells grown ± unsaturated fatty acids. Strain W303-1A was grown overnight and refreshed into YNBt medium with either 1% tergitol or 0.1% tergitol, containing 1 mM noted fatty acid, or ethanol for control, for four hours. Cells were harvested, fatty acid methyl esters prepared and analyzed by GC as described in Section 2. At least three replicates were analyzed for each growth condition. Fatty acid profiles of cells grown in the presence or absence of unsaturated fatty acid supplements in either (a) 1% tergitol or (b) 0.1% tergitol. (c) A comparison of the relative amount of fed UFA present in 1% and 0.1% tergitol culture conditions.

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(b)

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