A Transcriptional Regulatory System of the S. cerevisiae OLE1 Gene Responds to Fatty Acid Species and Intracellular Amount, and not Simply Membrane Status

Figure 2

Effect of UFA species and tergitol concentration growth conditions on OLE1 expression. Wild type strain W303-1A with plasmid p62 (OLE1 promoter-lacZ reporter) was grown overnight, and refreshed into YNBt medium with either 1% tergitol or 0.1% tergitol, containing 1 mM noted fatty acid, or 1% ethanol for control, for four hours. Cells were harvested and β-galactosidase activity determined. Activity was normalized to ethanol control. At least 5 replicates were performed for each growth condition.