The Impacts of Ex Situ Transplantation on the Physiology of the Taiwanese Reef-Building Coral Seriatopora hystrix
Figure 3
TRIzol versus RNALater. Six Seriatopora hystrix samples were collected from Houwan, and three biopsies (50 mg) were removed from each and immersed in either TRIzol or RNALater as described in the text. Half of the samples stored in RNALater were homogenized and stored at −20°C, while the other half of the samples were simply kept at 4°C. RNA, DNA, and, in certain cases, protein were extracted from the six biopsies of each fixation strategy treatment. RNA concentration ([RNA]; black triangles and left -axis) and 260/280 (hollow circles and right -axis) and 260/230 (exes and right -axis) ratios were compared between the three fixation strategies (a). Likewise, the DNA concentration ([DNA]; black triangles and left -axis) and 260/280 (hollow circles and right -axis) and 260/230 (exes and right -axis) ratios were also compared (b). In panel b, the lower case letters adjacent to icons represent Tukey’s honestly significant difference (HSD) groups () for the [DNA] data, while upper case letters represent HSD groups for the 260/230 ratio data. Both protein concentration ([protein]; black circles connected by a dotted line and left -axis) and total protein (hollow squares connected by a solid line and right -axis) were compared between only two fixation strategies, immersion in either TRIzol or RNALater (without homogenization) (c). In all panels, error bars represent standard error of the mean.
