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Journal of Marine Biology
Volume 2016, Article ID 3683809, 11 pages
http://dx.doi.org/10.1155/2016/3683809
Research Article

Adverse Effects of Immobilised Pseudoalteromonas on the Fish Pathogenic Vibrio anguillarum: An In Vitro Study

1Mikrobiologisches Labor Dr. Michael Lohmeyer GmbH, Mendelstraße 11, 48149 Münster, Germany
2Advanced Ceramics, University of Bremen, Am Biologischen Garten 2, 28359 Bremen, Germany
3MAPEX Center for Materials and Processes, Bibliothekstraße 1, 28359 Bremen, Germany
4Novelpor UG, Huchtinger Heerstraße 47, 28259 Bremen, Germany
5Leibniz-Center for Tropical Marine Ecology GmbH, Fahrenheitstraße 6, 28359 Bremen, Germany
6AquaCare GmbH & Co. KG, Am Wiesenbusch 11, 45966 Gladbeck, Germany
7Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstraße 3, 48149 Münster, Germany

Received 7 April 2016; Revised 5 July 2016; Accepted 28 July 2016

Academic Editor: Horst Felbeck

Copyright © 2016 Wiebke Wesseling et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

As a prerequisite for use in marine aquaculture, two immobilisation systems were developed by employing the probiotic bacterium Pseudoalteromonas sp. strain MLms_gA3. Their impact on the survivability of the fish pathogen Vibrio anguillarum was explored. Probiotic bacteria either grown as a biofilm on ceramic tiles or embedded in alginate beads were added to sterile artificial seawater that contained the fish pathogen. While immobilisation on ceramics followed a recently developed protocol, a medium allowing for alginate microencapsulation was newly developed. Anti-Vibrio activities were obtained with both immobilisation systems. The viable cell counts of V. anguillarum constantly decreased within the first two weeks of the treatments evidencing the potential of the immobilisation systems for providing probiotic-based protection against this pathogen.