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Journal of Marine Biology
Volume 2016, Article ID 3683809, 11 pages
Research Article

Adverse Effects of Immobilised Pseudoalteromonas on the Fish Pathogenic Vibrio anguillarum: An In Vitro Study

1Mikrobiologisches Labor Dr. Michael Lohmeyer GmbH, Mendelstraße 11, 48149 Münster, Germany
2Advanced Ceramics, University of Bremen, Am Biologischen Garten 2, 28359 Bremen, Germany
3MAPEX Center for Materials and Processes, Bibliothekstraße 1, 28359 Bremen, Germany
4Novelpor UG, Huchtinger Heerstraße 47, 28259 Bremen, Germany
5Leibniz-Center for Tropical Marine Ecology GmbH, Fahrenheitstraße 6, 28359 Bremen, Germany
6AquaCare GmbH & Co. KG, Am Wiesenbusch 11, 45966 Gladbeck, Germany
7Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstraße 3, 48149 Münster, Germany

Received 7 April 2016; Revised 5 July 2016; Accepted 28 July 2016

Academic Editor: Horst Felbeck

Copyright © 2016 Wiebke Wesseling et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The Supplementary Material presents at first one of the alginate beads loaded with potential probiotic Pseudoalteromonas sp. MLms_gA3 cells (figure S1), which were applied towards V. anguillarum cells (for results see section 3.3). A biofilm formation of V. anguillarum on a ceramic tile which was incubated for 28 days in sterilised seawater was not detected by SEM-analysis (figure S2, right). Nevertheless, a formation of V. anguillarum cell aggregates in the sea water was not excluded. The sea water was inoculated with precultures of Pseudoalteromonas sp. MLms_gA3 and V. anguillarum. The optical density of the precultures with the associated viable cell counts was determined to adjust the desired cell concentrations of Pseudoalteromonas sp. MLms_gA3 and V. anguillarum in the test tubes with sterilised sea water (table 1).

  1. Supplementary Material