Table of Contents Author Guidelines Submit a Manuscript
Journal of Nucleic Acids
Volume 2010, Article ID 421803, 3 pages
Research Article

Preparation of DNA Ladder Based on Multiplex PCR Technique

Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Henan 453003, China

Received 14 February 2010; Revised 26 April 2010; Accepted 11 May 2010

Academic Editor: Ashis Basu

Copyright © 2010 Tian-Yun Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. L. J. Wei, Y. T. Wei, X. F. Huang, X. Q. Wei, and R. B. Huang, “Construction of a new kind of DNA marker vector,” Biotechnology, vol. 14, no. 5, pp. 33–35, 2004. View at Google Scholar
  2. C. J. Zhu, Q. Y. Wei, W. L. Qu, and H. Ding, “Experimental study on making a new DNA marker from positive and negative DNA samples,” Acta Medicinae Sinica, vol. 18, pp. 937–938, 2005. View at Google Scholar
  3. Hyman, “DNA ladders,” US Patent no. 5,840,575, 1999.
  4. A-li W. Hu, J. L. Hartley, and H. J. Jordan, “Nucleic acid ladders,” US Patent no. 6,924,098, 2005.
  5. J. L. Hartley, “Nucleic acid marker ladder for estimating mass,” US Patent no. 7,132,520, 2006.
  6. Dawson, “Method for the multiplexed preparation of nucleic acid molecular weight markers and resultant products,” US Patent no. 5,714,326, 1998.
  7. D. J. Korbie and J. S. Mattick, “Touchdown PCR for increased specificity and sensitivity in PCR amplification,” Nature Protocols, vol. 3, no. 9, pp. 1452–1456, 2008. View at Publisher · View at Google Scholar · View at Scopus
  8. I. Smolina, C. Lee, and M. Frank-Kamenetsk II, “Detection of low-copy-number genomic DNA sequences in individual bacterial cells by using peptide nucleic acid-assisted rolling-circle amplification and fluorescence in situ hybridization,” Applied and Environmental Microbiology, vol. 73, no. 7, pp. 2324–2328, 2007. View at Publisher · View at Google Scholar · View at Scopus