Journal of Nucleic Acids / 2011 / Article / Fig 2

Review Article

Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA

Figure 2

Specific chemical reactions for m3C and m5C detection. (a) Cleavage of m3C and unmodified uridine by hydrazine. (b) Deamination of unmodified cytosine by bisulfate; m5C is resistant for deamination. (c) Specific oxydation of 5-6 double C=C bond in methylated pyrimidines by , OsO4, and OsO4 derivatives.

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