Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA
Specific chemical reactions for m3C and m5C detection. (a) Cleavage of m3C and unmodified uridine by hydrazine. (b) Deamination of unmodified cytosine by bisulfate; m5C is resistant for deamination. (c) Specific oxydation of 5-6 double C=C bond in methylated pyrimidines by , OsO4, and OsO4 derivatives.
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