Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA
Figure 7
Specific reactivity of free NH2– (a) and –COOH (b) groups in modified nucleotides. acp3U is taken to illustrate the reactivity with isothiocyanate derivatives and NHS derivatives. Reaction with dansyl chloride is shown for preQ1 (a). Reactions of free –COOH groups with aniline, ethylenediamine and similar molecules in the presence of soluble carbodiimide. The resulting free NH2-group of ethylenediamine may be further used for attachment of activated acyl (b). The structure of mt6A(m6t6A) is shown at the bottom.
