Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA

Figure 8

Cleavage of the dihydrouridine ring (a) upon reduction by sodium borohydride (NaBH₄) and (b) at mild alkaline conditions. In both cases, cleavage of the dihydrouridine ring is followed by cleavage of the RNA chain.

(a)

(b)