Site-Selective Artificial Ribonucleases: Oligonucleotide Conjugates Containing Multiple Imidazole Residues in the Catalytic Domain

Figure 1

Schematic representation of the catalytic structures of the site-selective artificial ribonucleases B-Im(N/m) and analysis of the conjugates. (a) Anchor groups used for attachment of dendrimeric RNA-cleaving constructs to oligonucleotide B 5′-phosphate. (b) Schematic representation of conjugates bearing four and eight imidazole residues and anchor groups of Type1, Type 2, and Type 3, obtained using precursor technology. Mass spectrometry data: 1: calculated mass 5523.30, found mass 5523.73; 2: 5566.80/5566.39; 3: 5692.89/5692.03; 4a: 6838.95/6838,65; 5: 6423.63/6425.03. (c) Analysis of the conjugates B-Im(N/m) by 12% polyacrylamide/8 M urea gel electrophoresis. 0.1 A₂₆₀ unit of each conjugate was applied on the gel; the conjugates were visualized in the gel by staining with Stains-All. Numbers on the top of the gel correspond to numeration of the conjugates in the Table 1 and Figure 1(b) and Supplementary Material Figure  1.