Identification of the DNA-Binding Domains of Human Replication Protein A That Recognize G-Quadruplex DNA

Figure 5

FP ssDNA binding assays for RPA-CDE-core, RPA-DE and RPA-C at 10 mM NaCl. Representative binding isotherms are shown. (a)–(d) RPA-CDE-core, (e)–(h) RPA-DE, and (i)–(l) RPA-C. The layout of the figure is the same as described in Figure 4. All experiments were performed at a lowered NaCl concentration to observe binding by the low-affinity RPA constructs and for comparison with RPA-CDE-core.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)