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Journal of Nucleic Acids
Volume 2012 (2012), Article ID 392039, 8 pages
Research Article

Structural and Functional Characterization of RecG Helicase under Dilute and Molecular Crowding Conditions

1Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan
2Amity Institute of Biotechnology, Amity University Uttar Pradesh, Sector-125, Expressway Highway, Noida 201303, India
3Department of Nanobiochemistry, Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan

Received 4 April 2012; Accepted 30 May 2012

Academic Editor: Masayasu Kuwahara

Copyright © 2012 Sarika Saxena et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

A 62-mer DNA oligonucleotide was designed to form a frayed DNA duplex containing non-complementary arms and stem region. This arrangement of bases within the target duplex mimics a replication fork. Truncated sequence constituting only the stem region was also studied separately under similar conditions.

  1. Supplementary Martrial