Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage

Figure 2

Top: western analysis of PCNA protein levels in total cell extracts of the CL1-0 cells stably transfected with wild type PCNA (EGFP-wt. PCNA) or truncated PCNA (EGFP-) expression plasmids or EGFP-vector alone. Bottom: overexpression of PCNA attenuates colcemid-caused delay of gap-filling. CL1-0 cells stably transfected with the indicated plasmids were treated with the procedures illustrated in Figure 1 for studying the effect of PCNA overexpression on colcemid-caused delay of gap-filling during repair of UV-induced DNA lesions.