Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage

Figure 8

The effect of XRCC1 overexpressed on the gap-filling during repair of UV-induced DNA lesions. ((a) and (b)) Similar to the procedures described in Figures 1 and 3, yet colcemid was excluded. (a) AGS cells were transiently transfected with pCMV XRCC1 plasmids (open circle) or not (closed circle). (b) Cont. for control, that is, cells without treatment. AGS cells were transfected with wt. PCNA or pCMV-XRCC1 or empty vector. Time points: 0–8 h after H/A removal. Histograms of both panels: -axis: forward scattering (relevant to particle size); -axis: cell counts. (c) Similar to procedures described in Figure 1, colcemid was present, and AGS cells transfected with empty vector or plasmid EGF-PCNA with or without pCMV XRCC1 were used.

(a)

(b)

(c)