Genetic Clearness Novel Strategy of Group I Bacillus Species Isolated from Fermented Food and Beverages by Using Fibrinolytic Enzyme Gene Encoding a Serine-Like Enzyme

Table 1

Primers used in this study in terms of identification of strains.

Oligos names	5′- - - -3′	Size	Targeted species
	For Group 1 Bacillus Identification
Ba.IdMa-F	GCGCAGTCCGTGCCTTACGGCGT	828 pb	B. amyloliquefaciens
Ba.IdMa-R	TTACTGAGCTGCCGCCTGTACG
Ba.SHMa-F	TACAACTCTCACGGAACTCACGTTGCC	461 pb
Ba.SHMa-R	TATTTGTTTCCAGGAAGCGTG
Bl.Id.Ma-F	GCGCAAACCGTTCCTTACGGCAT	825pb	B. licheniformis
Bl.Id.Ma-R	TTATTGAGCGGCAGCTTCGAC
Bl.SHMa-F	GGCAACGGACACGGCACACATGTTGC	461 pb
Bl.SHMa-R	TAAGTGTTCGTTGGGTAAGTG
Bs.Id.Ma-F	GCGCAATCTGTTCCTTATGGCAT	835 pb	B. subtilis
Bs.Id.Ma-R	TTATTGTGCAGCTGCTTGTACGTTGA
Bs.SHMa-F	GGCAGTTCTCACGGTACGCATGTAGCC	461 pb
Bs.SHMa-R	TAAGTGCCTCCAGGAAGTGTG
Bp.Id.Ma-F	GCACAAACCGTCCCTTATGGAAT	828 pb	B. pumilus
Bp.Id.Ma-R	TTAGTTAGAAGCCGCTTGAGCG
Bp.SHMa-F	TTTCAATCACATGGAACTCACGTAGCC	461 pb
Bp.SHMa-R	TATCCACTGCTTGGTACTGTA
Bm.Id.Ma-F	GCGCAATCTGTTCCTTACGGCAT	837 pb	B. mojavensis
Bm.Id.Ma-R	TTATTGTGCAGCTGCCTGCAC
Bm.SHMa-F	GGCAGTTCTCACGGCACGCATGTAGCC	461 pb
Bm.SHMa-R	TATGTGCCGCCAGGAAGTGTG
Bsa.Id.Ma-F	GCACAAACCGTCCCTTATGGAAT	828 pb	B. safensis
Bsa.Id.Ma-R	TTAGTTAGAAGCCGCTTGAACGTTG
Bsa.SHMa-F	TTTCAATCACATGGAACTCACGTAGCA	461 pb
Bsa.SHMa-R	TATCCACTGCTTGGCACTGTA
Bat.Id.Ma-F	GCTCAGTCA GTACCTTATG GCAT	828 pb	B. atrophaeus
Bat.Id.Ma-R	TTATTGCGCTGCTGCCTGAACG
Bat.SHMa-F	GGAAATTCACACGGAACTCACG	461 pb
Bat.SHMa-R	TAGCTGCTGCCCGGAAGTGTG
Bal.Id.Ma-F	GGTCAAAGCGTCCCTTATGGTA	828 pb	B. altitudinis
Bal.Id.Ma-R	TTATCGTGCAGCTTTTTGTAC
Bal.SHMa-F	CCGCATGAACACGGAACCCACG	461 pb
Bal.SHMa-R	TATTCGTTATGAGGAATGGTA