DNA Ligase IV Prevents Replication Fork Stalling and Promotes Cellular Proliferation in Triple Negative Breast Cancer
Lig4 knockdown causes decline in cell numbers without enhancing apoptosis. (a, b) Lig4 depleted or control (NT) cells were counted every 24 hr for up to 120 hr. Lines are the linear regression (p<0.05; p<0.001, n=3). (c) Levels of apoptotic markers cleaved caspase-3 and 9 were assessed using western blot in Lig4 depleted and control cells treated with either replication toxins (1 μM doxorubicin, 2 mM hydroxyurea, 10 μM etoposide, or 0.2 μM camptothecin) or DMSO.