DNA Ligase IV Prevents Replication Fork Stalling and Promotes Cellular Proliferation in Triple Negative Breast Cancer
Lig4 depletion alone increased persistence of DNA double strand breaks. BT549 cells were transfected with siRNA targeting Lig4 or scrambled control (NT) followed by immunostaining for γH2AX foci. γH2AX foci were quantified using ImageJ software with DAPI stained area defined as region of interest per image and scored. Mean intensity signal of γH2AX was normalized to the DAPI stained area per image ( p<0.001).