Nanocrystalline Diamond as a Coating for Joint Implants: Cytotoxicity and Biocompatibility Assessment

Figure 5

Time-course behaviour of human bone marrow osteoblast cell cultures grown over NCD-coated Si₃N₄ substrates at 7 days ((a), (d)), 14 days (b), (e), and 21 days (c), (f). CLSM: (phalloidin and propidium iodide labelling, (a), (b); plus calcein staining, (c) and SEM (d)–(f) photographs. For comparison, the cell behaviour observed on standard polystyrene culture plates is also shown (SEM): (g), (h), (i), respectively, for 7, 14, and 21 days).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)