Suppression of Proinflammatory Cytokines in Functionalized Fullerene-Exposed Dermal Keratinocytes
Evaluation of apoptotic response in HEK cells. (a) Cells were treated with fullerene and its derivatives for 24 hours. Apoptotic cell death was quantified by photometric enzyme-immunoassay. Media and 1 M camptothecin (dash line) were used as negative and positive controls, respectively. (b) Cells were pretreated with fullerene and its derivatives for 2 hours, then cotreated with 1 M camptothecin for 24 hours. Relative change in apoptosis was compared to untreated cells (Media, dash line), and camptothecin (CAM). Values are presented as means SEM of triplicate cultures from two individual samples in each treatment group.