Table of Contents Author Guidelines Submit a Manuscript
Journal of Nanomaterials
Volume 2010 (2010), Article ID 437219, 7 pages
http://dx.doi.org/10.1155/2010/437219
Research Article

The 3-D Culture and In Vivo Growth of the Human Hepatocellular Carcinoma Cell Line HepG2 in a Self-Assembling Peptide Nanofiber Scaffold

Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Chengdu, 610041, China

Received 24 March 2010; Revised 1 August 2010; Accepted 13 August 2010

Academic Editor: Jun Liu

Copyright © 2010 Min Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. C. M. Perou, T. Sørile, M. B. Eisen et al., “Molecular portraits of human breast tumours,” Nature, vol. 406, no. 6797, pp. 747–752, 2000. View at Publisher · View at Google Scholar · View at Scopus
  2. D. T. Ross and C. M. Perou, “A comparison of gene expression signatures from breast tumors and breast tissue derived cell lines,” Disease Markers, vol. 17, no. 2, pp. 99–109, 2001. View at Google Scholar · View at Scopus
  3. F. Xu and K. J. L. Burg, “Three-dimensional polymeric systems for cancer cell studies,” Cytotechnology, vol. 54, no. 3, pp. 135–143, 2007. View at Publisher · View at Google Scholar · View at Scopus
  4. T. C. Holmes, S. de Lacalle, X. Su, G. Liu, A. Rich, and S. Zhang, “Extensive neurite outgrowth and active synapse formation on self-assembling peptide scaffolds,” Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 12, pp. 6728–6733, 2000. View at Google Scholar · View at Scopus
  5. C. E. Semino, J. R. Merok, G. G. Crane, G. Panagiotakos, and S. Zhang, “Functional differentiation of hepatocyte-like spheroid structures from putative liver progenitor cells in three-dimensional peptide scaffolds,” Differentiation, vol. 71, no. 4-5, pp. 262–270, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. R. G. Ellis-Behnke, Y.-X. Liang, S.-W. You et al., “Nano neuro knitting: peptide nanofiber scaffold for brain repair and axon regeneration with functional return of vision,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 13, pp. 5054–5059, 2006. View at Publisher · View at Google Scholar · View at Scopus
  7. H. Meng, L. Chen, Z. Ye, S. Wang, and X. Zhao, “The effect of a self-assembling peptide nanofiber scaffold (peptide) when used as a wound dressing for the treatment of deep second degree burns in rats,” Journal of Biomedical Materials Research—Part B, vol. 89, no. 2, pp. 379–391, 2009. View at Publisher · View at Google Scholar · View at Scopus
  8. M. Bokhari, R. J. Carnachan, N. R. Cameron, and S. A. Przyborski, “Novel cell culture device enabling three-dimensional cell growth and improved cell function,” Biochemical and Biophysical Research Communications, vol. 354, no. 4, pp. 1095–1100, 2007. View at Publisher · View at Google Scholar · View at Scopus
  9. R. Kokenyesi, K. P. Murray, A. Benshushan, E. D. Huntley, and M.-S. Kao, “Invasion of interstitial matrix by a novel cell line from primary peritoneal carcinosarcoma, and by established ovarian carcinoma cell lines: role of cell-matrix adhesion molecules, proteinases, and E-cadherin expression,” Gynecologic Oncology, vol. 89, no. 1, pp. 60–72, 2003. View at Publisher · View at Google Scholar · View at Scopus
  10. X. Zhao and S. Zhang, “Designer self-assembling peptide materials,” Macromolecular Bioscience, vol. 7, no. 1, pp. 13–22, 2007. View at Publisher · View at Google Scholar · View at Scopus
  11. J. Guo, H. Su, Y. Zeng et al., “Reknitting the injured spinal cord by self-assembling peptide nanofiber scaffold,” Nanomedicine, vol. 3, no. 4, pp. 311–321, 2007. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Zhanga, Y. Lin, M. Altman et al., “Biological surface engineering: a simple system for cell pattern formation,” Biomaterials, vol. 20, no. 13, pp. 1213–1220, 1999. View at Publisher · View at Google Scholar · View at Scopus
  13. C. Fischbach, R. Chen, T. Matsumoto et al., “Engineering tumors with 3D scaffolds,” Nature Methods, vol. 4, no. 10, pp. 855–860, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. N. Navarro-Alvarez, A. Soto-Gutierrez, J. D. Rivas-Carrillo et al., “Self-assembling peptide nanofiber as a novel culture system for isolated porcine hepatocytes,” Cell Transplantation, vol. 15, no. 10, pp. 921–927, 2006. View at Publisher · View at Google Scholar · View at Scopus
  15. S. Wang, D. Nagrath, P. C. Chen, F. Berthiaume, and M. L. Yarmush, “Three-dimensional primary hepatocyte culture in synthetic self-assembling peptide hydrogel,” Tissue Engineering Part A, vol. 14, no. 2, pp. 227–236, 2008. View at Publisher · View at Google Scholar · View at Scopus
  16. C. S. Ranucci, A. Kumar, S. P. Batra, and P. V. Moghe, “Control of hepatocyte function on collagen foams: sizing matrix pores toward selective induction of 2-D and 3-D cellular morphogenesis,” Biomaterials, vol. 21, no. 8, pp. 783–793, 2000. View at Publisher · View at Google Scholar · View at Scopus
  17. C. S. Ranucci and P. V. Moghe, “Polymer substrate topography actively regulates the multicellular organization and liver-specific functions of cultured hepatocytes,” Tissue Engineering, vol. 5, no. 5, pp. 407–420, 1999. View at Publisher · View at Google Scholar · View at Scopus
  18. R. Sudo, T. Mitaka, M. Ikeda, and K. Tanishita, “Reconstruction of 3D stacked-up structures by rat small hepatocytes on microporous membranes,” The FASEB Journal, vol. 19, no. 12, pp. 1695–1697, 2005. View at Publisher · View at Google Scholar · View at Scopus
  19. H. Baharvand, S. M. Hashemi, S. K. Ashtiani, and A. Farrokhi, “Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro,” International Journal of Developmental Biology, vol. 50, no. 7, pp. 645–652, 2006. View at Publisher · View at Google Scholar · View at Scopus
  20. S. Even-Ram and K. M. Yamada, “Cell migration in 3D matrix,” Current Opinion in Cell Biology, vol. 17, no. 5, pp. 524–532, 2005. View at Publisher · View at Google Scholar · View at Scopus
  21. B. A. Roeder, K. Kokini, J. E. Sturgis, J. P. Robinson, and S. L. Voytik-Harbin, “Tensile mechanical properties of three-dimensional type I collagen extracellular matrices with varied microstructure,” Journal of Biomechanical Engineering, vol. 124, no. 2, pp. 214–222, 2002. View at Publisher · View at Google Scholar · View at Scopus
  22. F. Gelain, A. Lomander, A. L. Vescovi, and S. Zhang, “Systematic studies of a self-assembling peptide nanofiber scaffold with other scaffolds,” Journal of Nanoscience and Nanotechnology, vol. 7, no. 2, pp. 424–434, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. M. S. Kim, J. H. Yeon, and J.-K. Park, “A microfluidic platform for 3-dimensional cell culture and cell-based assays,” Biomedical Microdevices, vol. 9, no. 1, pp. 25–34, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. A. Horii, X. Wang, F. Gelain, and S. Zhang, “Biological designer self-assembling peptide nanofiber scaffolds significantly enhance osteoblast proliferation, differentiation and 3-D migration,” PLoS ONE, vol. 2, no. 2, article e190, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. S. Wang, D. Nagrath, P. C. Chen, F. Berthiaume, and M. L. Yarmush, “Three-dimensional primary hepatocyte culture in synthetic self-assembling peptide hydrogel,” Tissue Engineering Part A, vol. 14, no. 2, pp. 227–236, 2008. View at Publisher · View at Google Scholar · View at Scopus