Application of Quantum Dots-Based Biotechnology in Cancer Diagnosis: Current Status and Future Perspectives

Figure 3

In vivo targeting and imaging of lung metastasis model with QD-AFP-Ab probes. (a) and (b) As compared with the control group (a), the whole body imaging showed that the QD fluorescence was localized in the lung metastases of the model (b). (c, d) As compared with the control group (c), the lung-imaging also showed that there was bright fluorescence in the lung metastases (d). (e) Microphotography of tumor metastasis to lungs, replacing the normal lung tissue with massive tumor cells obviously (HE stain, 200). (f) The confocal microscopic imaging of tumor metastasis to lungs, which showed the specific binding of QD-AFP-Ab probes to tumor cells. Scale bar: 20 mm. (g) and (h) The spectra analysis of the test group (g) and the control group (h) showed that the fluorescence spectra of metastases in the test group were the same with those of QD-AFP-Ab probes, but there was no characteristic 590 nm peak of the QD at those in the control group. (Work from [77].)