Quaternary Ammonium Polyethyleneimine: Antibacterial Activity

Table 2

Characterization of QA- PEI nanoparticles prepared via reductive amination and N-alkylation.

Code	Degree of alkylationa	Inhibition concentrationb	Elemental analysisc			C/Nd	Particlesize   (nm)e	Zeta potential (mV)f	Primary amineg
			%N	%C	%I
A	1 : 1	80 𝜇g/mL	7.03	32.74	54.00	3.61	24 ± 10	85 ± 10	3.21 * 10^-4
I	1 : 1	80 𝜇g/mL	7.24	35.38	43.16	3.56	40 ± 18	63 ± 27	3.37 * 10^-4

a,c-f See notes in Table 1.
b Concentration of the tested compound needed for a complete inhibition of >10⁶  S. aureus growth.
g Amount of primary amine (mmol/g) in conjugates determined by the TNBS method following alkylation step, while prior alkylation primary amine of PEI was 5.8 mmol/g.