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Journal of Nanomaterials
Volume 2011, Article ID 265251, 9 pages
http://dx.doi.org/10.1155/2011/265251
Research Article

Dependence of Spreading and Differentiation of Mesenchymal Stem Cells on Micropatterned Surface Area

1Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
2Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8571, Japan
3International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

Received 16 October 2010; Revised 6 January 2011; Accepted 5 February 2011

Academic Editor: Yanan Du

Copyright © 2011 Wei Song et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. F. M. Watt and B. L. M. Hogan, “Out of eden: stem cells and their niches,” Science, vol. 287, no. 5457, pp. 1427–1430, 2000. View at Publisher · View at Google Scholar · View at Scopus
  2. M. P. Lutolf, P. M. Gilbert, and H. M. Blau, “Designing materials to direct stem-cell fate,” Nature, vol. 462, no. 7272, pp. 433–441, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. F. Guilak, D. M. Cohen, B. T. Estes, J. M. Gimble, W. Liedtke, and C. S. Chen, “Control of stem cell fate by physical interactions with the extracellular matrix,” Cell Stem Cell, vol. 5, no. 1, pp. 17–26, 2009. View at Publisher · View at Google Scholar · View at Scopus
  4. T. Hoshiba, N. Kawazoe, T. Tateishi, and G. Chen, “Development of stepwise osteogenesis-mimicking matrices for the regulation of mesenchymal stem cell functions,” Journal of Biological Chemistry, vol. 284, no. 45, pp. 31164–31173, 2009. View at Publisher · View at Google Scholar · View at Scopus
  5. T. Hoshiba, N. Kawazoe, T. Tateishi, and G. Chen, “Development of extracellular matrices mimicking stepwise adipogenesis of mesenchymal stem cells,” Advanced Materials, vol. 22, no. 28, pp. 3042–3047, 2010. View at Publisher · View at Google Scholar
  6. L. Guo, N. Kawazoe, Y. Fan et al., “Chondrogenic differentiation of human mesenchymal stem cells on photoreactive polymer-modified surfaces,” Biomaterials, vol. 29, no. 1, pp. 23–32, 2008. View at Publisher · View at Google Scholar · View at Scopus
  7. L. Guo, N. Kawazoe, T. Hoshiba, T. Tateishi, G. Chen, and X. Zhang, “Osteogenic differentiation of human mesenchymal stem cells on chargeable polymer-modified surfaces,” Journal of Biomedical Materials Research. Part A, vol. 87, no. 4, pp. 903–912, 2008. View at Publisher · View at Google Scholar · View at Scopus
  8. N. Jaiswal, S. E. Haynesworth, A. I. Caplan, and S. P. Bruder, “Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro,” Journal of Cellular Biochemistry, vol. 64, no. 2, pp. 295–312, 1997. View at Publisher · View at Google Scholar · View at Scopus
  9. M. F. Pittenger, A. M. Mackay, S. C. Beck et al., “Multilineage potential of adult human mesenchymal stem cells,” Science, vol. 284, no. 5411, pp. 143–147, 1999. View at Publisher · View at Google Scholar · View at Scopus
  10. K. Alberti, R. E. Davey, K. Onishi et al., “Functional immobilization of signaling proteins enables control of stem cell fate,” Nature Methods, vol. 5, no. 7, pp. 645–650, 2008. View at Publisher · View at Google Scholar · View at Scopus
  11. M. R. Doran, B. D. Markway, I. A. Aird et al., “Surface-bound stem cell factor and the promotion of hematopoietic cell expansion,” Biomaterials, vol. 30, no. 25, pp. 4047–4052, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. A. J. Engler, S. Sen, H. L. Sweeney, and D. E. Discher, “Matrix elasticity directs stem cell lineage specification,” Cell, vol. 126, no. 4, pp. 677–689, 2006. View at Publisher · View at Google Scholar · View at Scopus
  13. M. J. Dalby, N. Gadegaard, R. Tare et al., “The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder,” Nature Materials, vol. 6, no. 12, pp. 997–1003, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. R. McBeath, D. M. Pirone, C. M. Nelson, K. Bhadriraju, and C. S. Chen, “Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment,” Developmental Cell, vol. 6, no. 4, pp. 483–495, 2004. View at Publisher · View at Google Scholar · View at Scopus
  15. R. Peerani, B. M. Rao, C. Bauwens et al., “Niche-mediated control of human embryonic stem cell self-renewal and differentiation,” EMBO Journal, vol. 26, no. 22, pp. 4744–4755, 2007. View at Publisher · View at Google Scholar · View at Scopus
  16. J. T. Connelly, J. E. Gautrot, B. Trappmann et al., “Actin and serum response factor transduce physical cues from the microenvironment to regulate epidermal stem cell fate decisions,” Nature Cell Biology, vol. 12, no. 7, pp. 711–718, 2010. View at Publisher · View at Google Scholar · View at Scopus
  17. K. Bhadriraju and L. K. Hansen, “Extracellular matrix- and cytoskeleton-dependent changes in cell shape and stiffness,” Experimental Cell Research, vol. 278, no. 1, pp. 92–100, 2002. View at Publisher · View at Google Scholar · View at Scopus
  18. E. Szabo, T. Feng, E. Dziak, and M. Opas, “Cell adhesion and spreading affect adipogenesis from embryonic stem cells: the role of calreticulin,” Stem Cells, vol. 27, no. 9, pp. 2092–2102, 2009. View at Publisher · View at Google Scholar · View at Scopus
  19. G. Chen, N. Kawazoe, Y. Fan, Y. Ito, and T. Tateishi, “Grid pattern of nanothick microgel network,” Langmuir, vol. 23, no. 11, pp. 5864–5867, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. M. Théry, V. Racine, A. Pépin et al., “The extracellular matrix guides the orientation of the cell division axis,” Nature Cell Biology, vol. 7, no. 10, pp. 947–953, 2005. View at Publisher · View at Google Scholar · View at Scopus
  21. M. Théry, V. Racine, M. Piel et al., “Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 52, pp. 19771–19776, 2006. View at Publisher · View at Google Scholar · View at Scopus
  22. J. E. Gautrot, B. Trappmann, F. Oceguera-Yanez et al., “Exploiting the superior protein resistance of polymer brushes to control single cell adhesion and polarisation at the micron scale,” Biomaterials, vol. 31, no. 18, pp. 5030–5041, 2010. View at Publisher · View at Google Scholar · View at Scopus
  23. R. G. Thakar, Q. Cheng, S. Patel et al., “Cell-shape regulation of smooth muscle cell proliferation,” Biophysical Journal, vol. 96, no. 8, pp. 3423–3432, 2009. View at Publisher · View at Google Scholar · View at Scopus
  24. C. S. Chen, M. Mrksich, S. Huang, G. M. Whitesides, and D. E. Ingber, “Geometric control of cell life and death,” Science, vol. 276, no. 5317, pp. 1425–1428, 1997. View at Publisher · View at Google Scholar · View at Scopus
  25. L. Q. Wan, S. M. Kang, G. Eng et al., “Geometric control of human stem cell morphology and differentiation,” Integrative Biology, vol. 2, no. 7-8, pp. 346–353, 2010. View at Publisher · View at Google Scholar
  26. N. Kawazoe, L. Guo, M. J. Wozniak et al., “Adipogenic differentiation of mesenchymal stem cells on micropatterned polyelectrolyte surfaces,” Journal of Nanoscience and Nanotechnology, vol. 9, no. 1, pp. 230–239, 2009. View at Publisher · View at Google Scholar · View at Scopus
  27. H. Lu, L. Guo, M. J. Wozniak et al., “Effect of cell density on adipogenic differentiation of mesenchymal stem cells,” Biochemical and Biophysical Research Communications, vol. 381, no. 3, pp. 322–327, 2009. View at Publisher · View at Google Scholar · View at Scopus
  28. D. Falconnet, G. Csucs, H. Michelle Grandin, and M. Textor, “Surface engineering approaches to micropattern surfaces for cell-based assays,” Biomaterials, vol. 27, no. 16, pp. 3044–3063, 2006. View at Publisher · View at Google Scholar · View at Scopus
  29. Y. Arima, M. Kawagoe, M. Furuta, M. Toda, and H. Iwata, “Effect of swelling of poly(vinyl alcohol) layers on complement activation,” Biomaterials, vol. 31, pp. 6926–6933, 2010. View at Google Scholar
  30. J. Y. Karp, J. Yeh, G. Eng et al., “Controlling size, shape and homogeneity of embryoid bodies using poly(ethylene glycol) microwells,” Lab on a Chip, vol. 7, pp. 786–794, 2007. View at Google Scholar
  31. Y. Y. Choi, B. G. Chung, D. H. Lee, A. Khademhosseini, J. H. Kim, and S. H. Lee, “Controlled-size embryoid body formation in concave microwell arrays,” Biomaterials, vol. 31, pp. 4296–4303, 2010. View at Google Scholar
  32. R. Singhvi, A. Kumar, G. P. Lopez et al., “Engineering cell shape and function,” Science, vol. 264, no. 5159, pp. 696–698, 1994. View at Google Scholar · View at Scopus
  33. C. Y. Tay, H. Yu, M. Pal et al., “Micropatterned matrix directs differentiation of human mesenchymal stem cells towards myocardial lineage,” Experimental Cell Research, vol. 316, no. 7, pp. 1159–1168, 2010. View at Publisher · View at Google Scholar · View at Scopus
  34. J. P. Rodríguez, M. González, S. Ríos, and V. Cambiazo, “Cytoskeletal organization of human mesenchymal stem cells (MSC) changes during their osteogenic differentiation,” Journal of Cellular Biochemistry, vol. 93, no. 4, pp. 721–731, 2004. View at Publisher · View at Google Scholar · View at Scopus
  35. G. Yourek, M. A. Hussain, and J. J. Mao, “Cytoskeletal changes of mesenchymal stem cells during differentiation,” ASAIO Journal, vol. 53, no. 2, pp. 219–228, 2007. View at Publisher · View at Google Scholar · View at Scopus
  36. M. Guvendiren and J. A. Burdick, “The control of stem cell morphology and differentiation by hydrogel surface wrinkles,” Biomaterials, vol. 31, no. 25, pp. 6511–6518, 2010. View at Publisher · View at Google Scholar · View at Scopus
  37. P. Roca-Cusachs, J. Alcaraz, R. Sunyer, J. Samitier, R. Farré, and D. Navajas, “Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation,” Biophysical Journal, vol. 94, no. 12, pp. 4984–4995, 2008. View at Publisher · View at Google Scholar · View at Scopus
  38. M. Théry, A. Pépin, E. Dressaire, Y. Chen, and M. Bornens, “Cell distribution of stress fibres in response to the geometry of the adhesive environment,” Cell Motility and the Cytoskeleton, vol. 63, no. 6, pp. 341–355, 2006. View at Publisher · View at Google Scholar · View at Scopus
  39. K. A. Kilian, B. Bugarija, B. T. Lahn, and M. Mrksich, “Geometric cues for directing the differentiation of mesenchymal stem cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 11, pp. 4872–4877, 2010. View at Publisher · View at Google Scholar · View at Scopus
  40. S. A. Ruiz and C. S. Chen, “Emergence of patterned stem cell differentiation within multicellular structures,” Stem Cells, vol. 26, no. 11, pp. 2921–2927, 2008. View at Publisher · View at Google Scholar · View at Scopus