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Journal of Nanomaterials
Volume 2011 (2011), Article ID 308016, 10 pages
http://dx.doi.org/10.1155/2011/308016
Research Article

Optimization of the Preparation Process for an Oral Phytantriol-Based Amphotericin B Cubosomes

1School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 51006, China
2Department of Pharmaceutical Sciences, Gannan Medical College, Ganzhou, China
3GuangDong Medical College, Dongguan, China
4Anton-Paar, Shanghai, China
5GuangDong Research Center for Drug Delivery Systems, Guangzhou, China

Received 25 April 2011; Revised 25 June 2011; Accepted 26 June 2011

Academic Editor: Xing J. Liang

Copyright © 2011 Zhiwen Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In order to develop an oral formation of Amphotericin B (AmB) using phytantriol- (PYT-) based cubosomes with desirable properties, homogenization conditions were firstly investigated to determine their effects on the morphological and dimensional characteristics of cubosomes. Under the optimized homogenization conditions of 1200 bar for 9 cycles, cubosomes with reproducible, narrow particle size distribution and a mean particle size of 256.9 nm ± 4.9 nm were obtained. The structure of the dispersed cubosomes was revealed by SAXS (small-angle X-ray scattering) and Cryo-TEM (cryogenic transmission electron microscopy) as a bicontinuous cubic liquid crystalline phase with Pn3m geometry. To overcome the poor drug solubility and increase the drug-loading rate, a solubilization method was firstly used to develop cubosomes containing AmB. The encapsulation efficiency determined by HPLC assay was , and UV spectroscopy and stability studies in simulated gastric fluids further confirmed that AmB was successfully encapsulated in cubosomes.