Journal of Nanomaterials

Journal of Nanomaterials / 2011 / Article

Research Article | Open Access

Volume 2011 |Article ID 354793 |

Sougata Ghosh, Sumersing Patil, Mehul Ahire, Rohini Kitture, Amit Jabgunde, Sangeeta Kale, Karishma Pardesi, Jayesh Bellare, Dilip D. Dhavale, Balu A. Chopade, "Synthesis of Gold Nanoanisotrops Using Dioscorea bulbifera Tuber Extract", Journal of Nanomaterials, vol. 2011, Article ID 354793, 8 pages, 2011.

Synthesis of Gold Nanoanisotrops Using Dioscorea bulbifera Tuber Extract

Academic Editor: Rakesh Joshi
Received10 May 2011
Accepted12 Jul 2011
Published15 Sep 2011


Biosynthesis of metal nanoparticles employing plant extracts and thereby development of an environmentally benign process is an important branch of nanotechnology. Here, the synthesis of gold nanoparticles using Dioscorea bulbifera tuber extract (DBTE) as the reducing agent is reported. Field emission scanning electron microscopy (FESEM), energy-dispersive spectroscopy (EDX), X-ray diffraction (XRD), and UV-visible absorption spectroscopy confirmed the reduction of gold ions to AuNPs. The anisotropic nanoparticles consist of a mixture of gold nanotriangles, nanoprisms, nanotrapezoid, and spheres. The kinetics of particle formation was time dependent and was enhanced by the increase of temperature from 6°C to 50°C, the optimum being 50°C. The optimum concentration of chloroauric acid was found to be 1 mM. Complete reduction of the metal ions within 5 hours by DBTE highlights the development of a novel ecofriendly route of biological synthesis of gold nanoparticles. This is the first paper on synthesis of gold nanoparticles using DBTE.

1. Introduction

The size and shape of the nanoscale matters have a considerable effect on its physicochemical properties. Among the recently studied shapes and structures of gold nanoparticles, the noteworthy includes nanorings, nanoplates, dendrimer-like shapes, nanocubes, and nanoprisms [15]. The nonspherical gold nanoparticles possess unusual optical and electronic properties, improved mechanical properties, and specific surface enhance spectroscopies that make them ideal structures for emerging applications in photonics, electronics, optical sensing and imaging, biomedical labeling, and catalysis [611]. Red colloidal gold has been used as medicine for revitalization in China and India [12]. Gold nanoparticles have found use in biomedicinal applications particularly diagnostic and drug delivery [13]. There are a number of chemical methods for the synthesis of Au nanoparticles [1416] that may pose a risk due to adverse effects in medical applications rendered by presence of toxic chemical species adsorbed on the surface. Thus, currently there is a growing need to develop environmentally benign nanoparticle synthesis processes that are free from toxic chemicals in the synthesis protocols. Biosynthesis of nanoparticles employing microorganisms or plants can potentially eliminate this problem by making the nanoparticles more biocompatible. As a result, researchers in the field of nanoparticle synthesis and assembly have turned to biological systems such as yeast [17], fungi [1820], and bacteria [2123]. In recent years plant-mediated biological synthesis of nanoparticles is gaining importance due to its simplicity and ecofriendliness. Biosynthesis of Au nanoparticles by plants such as Magnolia kobus, Diospyros kaki [24], Azadirachta indica [25], Syzygium aromaticum [26], Medicago sativa [27], Aloe vera [28], and Cinnamomum camphora [29] have been reported.

Here in we report for the first time an environmentally friendly and rapid method for the aqueous synthesis and stabilization of gold nanoparticles by the reduction of aqueous AuCl4 ions using the aqueous extract of Dioscorea bulbifera tubers (DBTE). D. bulbifera belongs to the family Dioscoreaceae and the bulbs are profusely used in Indian and Chinese traditional medicine. It is reported to have antidiabetic [30, 31], anti-inflammatory [32], antitumor [33], and antimicrobial properties [34]. DBTE is rich in flavonoids and catechin that belongs to a class of polyphenolic compounds [35] that have contributed to its enhanced antioxidant properties [36]. However, to date, there have been no reports on the synthesis of Au nanoparticles with DBTE. We have used tuber extract for the biogenic reduction of Au3+ ions. We also investigated the effects of reaction conditions such as time course, reaction temperature, and concentration of chloroauric acid on the rate of synthesis of the gold nanoparticles.

2. Experimental

2.1. Plant Material and Preparation of Extract

Dioscorea bulbifera tubers (DBTs) were collected from Pune, chopped into thin slices, and dried for 2 days at room temperature under shade. Tuber extract was prepared by taking 5 g of thoroughly washed and finely ground tuber powder in a 300 mL Erlenmeyer flask with 100 mL of sterile distilled water and then boiling the mixture for 5 min before finally decanting it. The extract obtained was filtered through Whatman filter paper No. 1. The filtrate was collected and stored at 4°C for further use.

2.2. Synthesis of Gold Nanoparticles

Reduction of Au3+ ions was initiated by addition of 5 mL of DBTE to 95 mL of 10−3 M aqueous HAuCl4·3H2O solution in a 500 mL Erlenmeyer flask. The pH of the extract was found to be neutral. Thereafter, the flasks were shaken at a rotation rate of 150 rpm in the dark at 40°C. Reduction of the Au3+ ions was monitored by measuring the UV-Vis spectra of the solution at regular intervals on a UV-1650CP Schimadzu spectrophotometer operated at resolution of 1 nm. Effects of temperature on the rate of synthesis and particle size of the synthesized gold nanoparticles were studied by carrying out the reaction in water bath at 4–50°C with reflux. Concentration of chloroauric acid in the solution was also varied from 0.3–5 mM.

2.3. Field Emission Scanning Electron Microscope (FESEM) and Dynamic Light Scattering (DLS) Measurements

Surface morphology and particle size of bioreduced gold nanoparticles were determined using a field emission scanning electron microscope (Hitachi S-4800) equipped with energy dispersive spectrometer (EDS). Energy dispersive spectra of gold nanoparticles were taken in the same instrument at an energy range 0–20 keV confirmed the synthesis of Au nanoparticles using DBTE. The size of particles in the 3 mL of reaction mixture was analyzed by using the dynamic light scattering equipment (Malvern: Zetasizer Nano-2590) in a polysterene cuvettes.

2.4. X-Rray Diffraction (XRD) Measurements

The phase formation of bioreduced gold nanoparticles was studied with the help of XRD. The diffraction data of thoroughly dried thin films of nanoparticles on glass slides was recorded on D 8 Advanced Brucker X-ray diffractometer with Cu Kα (1.54 Å) source.

2.5. Fourier Transform Infrared (FTIR) Spectroscopy

Dry powder of nanoparticle was obtained in the following manner. The Au nanoparticles synthesized after 90 min of reaction of 1 mM HAuCl4·3H2O solution with the DBTE were centrifuged at 10,000 rpm for 15 min at room temperature, following which the pellet was redispersed in sterile distilled water to remove any uncoordinated biological molecules. The process of centrifugation and redispersion in sterile distilled water was repeated three times to ensure better separation of free entities from the nanoparticles. The purified pellet was then dried and subjected to FTIR (Shimadzu IR Affinity) spectroscopy measurement using the potassium bromide (KBr) pellet technique in the diffused reflection mode at a resolution of 4 cm−1. Au nanoparticle powder was mixed with KBr and subjected to IR source 500 cm−1–4000 cm−1. Similar process was used for the FTIR study of DBTE before and after bioreduction.

3. Results and Discussion

3.1. Visual Observation and UV-Vis Spectroscopy

It is reported that DBTE contains myrecetin and catechin as well as important terpenes like neoxanthin and lutein that may be responsible for reduction of metal ions by the oxidation of –OH groups in the molecules to carboxylic acid [32]. The stabilization may be due to the starch present in the extract which keeps the particles well separated. Colloidal solutions of Au nanoparticles showed a very intense ruby red color, which is absent in the bulk material as well as for individual atoms. Their origin is attributed to the collective oscillation of free conduction electrons induced by an interacting electromagnetic field.

These surface plasmon resonance contribute to the development of an array of colors to the reaction mixtures. The progress of the reaction between metal ions and the DBTE was monitored by recording the absorption spectra as a function of time. In each case peak was observed in the range of 500 to 600 nm. Figure 1 shows the result of the reaction between the Au3+ ion-containing solutions and the DBTE extract with time. Upon shaking at 40°C for 5 min, no subsequent change was observed but at 10 min a small peak appears at 540 nm., the absorbance steadily builds up with time. The maximum production was found at 90 min. The absorption peak is assigned to the surface plasmon resonance (SPR) band of Au nanoparticles formed by the reduction of Au3+ ions.

Optimization for concentration of chloroauric acid was carried on by plotting the peak absorbance at main peak wavelengths for Au nanoparticles against time. As shown in Figure 2, a variability in the reaction rate was observed with different concentrations of chloroauric acid. It is interesting to note that the absorbance increased steeply at early times, up to the first 90 min, after which the rate of change is reduced, and finally, a saturation was observed shortly after 100 min. A similar trend was followed by the concentrations ranging from 0.3 to 2 mM. However, in case of higher concentrations (3 mM to 5 mM) no increase in absorbance was observed. Optimization study showed a significant effect of concentration of chloroauric acid on the surface plasmon properties of the Au nanoparticles in Figure 2. Light grayish purple color was found to be developed in the reaction mixture after 5 min at 0.3 mM concentration. The yellow color of the gold chloride solution turned to be purplish pink at 0.5 mM followed by ruby red at 0.7 mM concentration. A dark brown color was obtained at 1 mM concentration. At higher concentration (2–5 mM) the property of the gold nanoparticles were found to be entirely different showing a shiny yellow color of metallic gold with deposition of the particles at the base. Our results thus demonstrate that a rapid biosynthesis of stable gold nanoparticles employing DBTE is brought about by the biogenic reduction of Au3+ ions the concentration optima of the gold chloride being 1 mM. The anisotropy was supported by the broadening of the peak in the UV-Vis scan of the reaction broths containing various concentrations of chloroaurate after 90 min of bioreduction by DBTE.

We further investigated the possibility of controlling the temperature conditions for maximal synthesis of Au nanoparticles by enhancement of the rate of the reaction. Figure 4 shows the effect of temperature on conversion of Au3+ to Au0. It showed a steady rise in the absorbance with development of intense red color with increase in the temperature. Although the reaction was not static at 4°C, its absorbance recorded even after 90 min was not significantly high denoting a partial reduction of the gold nanoparticles at lower temperature. Similarly, lower reaction rate with faint coloration of the reaction mixture at 10, 20, and 30°C confirmed the temperature dependence nature of the bioreduction by DBTE. The maximum conversion was observed at higher temperatures, optimum being 50°C.

3.2. Field Emission Scanning Electron Microscope-Energy Dispersive Spectrum (FSEM-EDS) Analysis

FESEM analysis revealed that the DBTE leads to the synthesis of anisotropic gold nanoparticles. The biosynthesized gold nanoparticles presented variable shape, most of them being spherical in nature with a few having occasionally triangular shape. This is in agreement with earlier reports on biological synthesis employing lemon grass [25]. The gold nanospheres formed predominantly with diameter ranging from 11 to 30 nm where maximum number of gold nanoparticles size was around 18 nm. Still, the actual occurrence of the nanoprisms and nano-trapezoids is an intriguing phenomenon which has rarely been observed for Au nanoparticles synthesized with the help of natural reducing agents like plant extracts. Figure 5(a) shows the image of Au nanoparticles produced by the DBTE. Although the majority of the particles appeared more or less spherical, nevertheless nanoprisms were observed here too.

Growth process, resulting in elongation of the gold seeds rather than formation of new gold nuclei leads to anisotropic gold nanoparticles with larger dimensions [3739]. Figure 5(b) shows distinct nanotriangle with equilateral edges, the length being approximately 270 nm. It has been reported that chloride ions are primarily responsible for the synthesis of gold triangles [40, 41]. The triangles were found to be well dispersed. Variation in the size of triangles was also observed which were in the range from 50 to 300 nm. The variation in size might be due to merging of large number of nuclei over the surface of the seed particles in the early stage of reaction. This kind of growth process is driven by very fast reduction rate of Au+ ion, assembly and room-temperature sintering of spherical gold nanoparticles. Although the dried biomass fulfilled the reduction of chloroaurate ions, most of the quasispherical nascent nanocrystals synthesized at a later stage aggregated due to deficiency of biomolecules used up during the course of reduction. However, the nanospherical particles produced at the initial stage of reaction were stable owing to the shelter of the protecting biomolecules.

It is important to note that D. bulbifera contains saponin like spiroconazole A as one of its major phytoconstituent with surfactant property [42]. Surfactants are known to spontaneously organize into micelles in aqueous media [43, 44]. These spatially and dimensionalized water pools can be used as soft templates to promote the formation of gold nanoparticles with various shapes and sizes [4548]. The anisotropy is evident in Figures 5(d), 5(e), and 5(f) that showed irregular shapes, especially Au nanotrapezoid and Au nanohexagons, respectively. Thus the saponins in D. bulbifera might play a major role as useful templates for generating nonspherical gold nanoparticles. Interestingly, nanospheres (18 nm) were found to adhere on the surface of the larger nanoparticles, namely, nanohexagons. It has been reported that as the solvent evaporates, capillary forces draw the nanospheres together, and the nanospheres crystallize into a hexagonally close packed pattern on the substrate [49]. Figure 5(d) shows the average size of the trapezoids to be 300 nm. Further study was carried out using EDX to confirm the element of the particle, and the EDX pattern obtained has been represented in Figure 6 that provided chemical analysis of the field view as well as spot analysis of the minute particles and confirmed the presence of specific elements.

The EDX analysis displayed signature spectra for gold convincingly evidenced the presence of gold in the polymorphic nanoparticles. These results are consistent with the EDS analysis data of gold structure synthesized by using extracts of Scutellaria barbata [50]. The particle size distribution of the gold nanoparticles determined by dynamic light scattering is shown in Figure 7 which was found to be in well agreement with FESEM analysis.

3.3. XRD Analysis

The bioreduced metal nanoparticle was confirmed as elemental Au using XRD. Figure 8 showed the XRD data of the bioreduced gold sample.

The obtained data matched with Joint Committee for Powder Diffraction Set (JCPDS) Data 04–0784 confirming the cubic phase of synthesized gold nanoparticles with lattice constant  Å. Since no other peaks than pure gold were observed in the diffraction data, the as-synthesized gold sample is pure. The inset shows peak broadening of the highest intensity peak corresponding to (111) plane, indicating the reduction in the crystallite size. The crystallite size was calculated using Scherrer’s formula [51]:

Where 0.9 is the shape factor, generally taken for a cubic system, λ is the X-ray source wavelength, typically 1.54 Å, β is the full width at half the maximum intensity (FWHM) in radians, and θ is the Bragg angle.

Using the above formula the crystallite size was calculated to be ~13 nm.

3.4. Fourier Transform Infrared Spectroscopy (FTIR) Analysis

D. bulbifera tubers are known to contain flavonoids [33], terpenoids [42, 52], phenanthrenes [53], amino acids [54], proteins [55, 56], and glycosides [57]. FTIR absorption spectra of the dried biomass of D.bulbifera tuber before and after bioreduction, as shown in Figure 9, offered information regarding the chemical change of the functional groups involved in bioreduction. The plant extract showed strong peak at ~3300 cm−1 which is a characteristic of hydroxyl group in polyphenolic compounds. The intensity of the peak (3100–3400 cm−1) corresponding to hydroxyl group in the plant extract was partially reduced during very slow reduction process at 4°C while significantly reduced (3580–3650 cm−1) after the completion of bioreduction under optimum conditions. This was accompanied by appearance of a new C=O carbonyl stretch from carboxylic acid at 1718 cm−1. Both the facts indicated involvement of hydroxyl group in the reduction of gold salt. The sharp peak at 1598 cm−1 represents N–H bond in –NH2 group. The presence of peaks corresponding to 3749 and 1523 cm−1 indicate that the gold nanoparticles are surrounded by amines since the peaks indicate –NH2 symmetric stretching and N–O bond in nitro compounds [58, 59]. Chen and Kimura had reported that the peaks 1540–1650 cm−1 and 1660–1450 cm−1 are characteristic of C=C [60]. The C–C is represented by the peak at 1022 cm−1. One of the significant peaks is at 582 cm−1 which shows chloroalkane, that is, C–Cl bond, indicating the bonding of the salt HAuCl4 to the plant extract through carbon-chlorine linkage/bond. As stated earlier, DBTE is mainly composed of flavonol glycosides and proteinaceous matter, which may play the key role in reduction, assembly, and stabilization of the gold nanoparticles

4. Conclusion

In conclusion, DBTE could be used as an efficient green material for the rapid and consistent synthesis of gold nanoparticles. The morphology of the particles formed consists of a mixture of gold nanoprisms, trapezoids, triangles, and spheres with face centered cubic (FCC) structure of gold (111). A variation in reaction conditions had pronounced effects on the reaction kinetics. This simple, low cost, nontoxic, and eco-friendly plant tuber could thus be used as an efficient alternative to the cost-intensive conventional methods. The gold nano-anisotrops generated by this nonconventional method could have variety of applications in the future. Moreover, this system could also be used as a model for understanding the mechanism of evolution of microstructure mediated by biological systems. The present study opens up a new possibility of very conveniently synthesizing Au nanoparticles using natural products which will be useful in optoelectronic and biomedical applications.


The authors are grateful to Dr. D. P. Amalnerkar, Executive Director, Centre for Materials for Electronics Technology (CMET), Pune, for FESEM measurements. S. Ghosh is thankful to University of Pune, Pune, and A. Jabgunde is thankful to UGC, New Delhi for providing research fellowship.


  1. G. S. Métraux, Y. C. Cao, R. Jin, and C. A. Mirkin, “Triangular nanoframes made of gold and silver,” Nano Letters, vol. 3, no. 4, pp. 519–522, 2003. View at: Publisher Site | Google Scholar
  2. X. Sun, S. Dong, and E. Wang, “High-yield synthesis of large single-crystalline gold nanoplates through a polyamine process,” Langmuir, vol. 21, no. 10, pp. 4710–4712, 2005. View at: Publisher Site | Google Scholar
  3. S. Pang, T. Kondo, and T. Kawai, “Formation of dendrimer-like gold nanoparticle assemblies,” Chemistry of Materials, vol. 17, no. 14, pp. 3636–3641, 2005. View at: Publisher Site | Google Scholar
  4. C. J. Huang, Y. H. Wang, P. H. Chiu, M. C. Shih, and T. H. Meen, “Electrochemical synthesis of gold nanocubes,” Materials Letters, vol. 60, no. 15, pp. 1896–1900, 2006. View at: Publisher Site | Google Scholar
  5. J. E. Millstone, S. Park, K. L. Shuford, L. Qin, G. C. Schatz, and C. A. Mirkin, “Observation of a quadrupole plasmon mode for a colloidal solution of gold nanoprisms,” Journal of the American Chemical Society, vol. 127, no. 15, pp. 5312–5313, 2005. View at: Publisher Site | Google Scholar
  6. D. Seo, C. I. Yoo, I. S. Chung, S. M. Park, S. Ryu, and H. Song, “Shape adjustment between multiply twinned and single-crystalline polyhedral gold nanocrystals: decahedra, icosahedra, and truncated tetrahedra,” Journal of Physical Chemistry C, vol. 112, no. 7, pp. 2469–2475, 2008. View at: Publisher Site | Google Scholar
  7. J. Aizpurua, P. Hanarp, D. S. Sutherland, M. Käll, G. W. Bryant, and F. J. G. de Abajo, “Optical properties of gold nanorings,” Physical Review Letters, vol. 90, no. 5, pp. 057401/1–057401/4, 2003. View at: Google Scholar
  8. D. H. Gracias, J. Tien, T. L. Breen, C. Hsu, and G. M. Whitesides, “Forming electrical networks in three dimensions by self-assembly,” Science, vol. 289, no. 5482, pp. 1170–1172, 2002. View at: Publisher Site | Google Scholar
  9. P. Gomez-Romero, “Hybrid organic-inorganic materials in search of synergic activity,” Advanced Materials, vol. 13, no. 3, pp. 163–174, 2001. View at: Publisher Site | Google Scholar
  10. H. Qiu, B. Rieger, R. Gilbert, and C. Jérôme, “PLA-coated gold nanoparticles for the labeling of PLA biocarriers,” Chemistry of Materials, vol. 16, no. 5, pp. 850–856, 2004. View at: Publisher Site | Google Scholar
  11. K. B. Narayanan and N. Sakthivel, “Coriander leaf mediated biosynthesis of gold nanoparticles,” Materials Letters, vol. 62, no. 30, pp. 4588–4590, 2008. View at: Publisher Site | Google Scholar
  12. R. Bhattacharya and P. Mukherjee, “Biological properties of “naked“ metal nanoparticles,” Advanced Drug Delivery Reviews, vol. 60, no. 11, pp. 1289–1306, 2008. View at: Publisher Site | Google Scholar
  13. M. Bartneck, H. A. Keul, S. Singh et al., “Rapid uptake of gold nanorods by primary human blood phagocytes and immunomodulatory effects of surface chemistry,” ACS Nano, vol. 4, no. 6, pp. 3073–3086, 2010. View at: Publisher Site | Google Scholar
  14. P. R. Selvakannan, S. Mandal, R. Pasricha, S. D. Adyanthaya, and M. Sastry, “One-step synthesis of hydrophobized gold nanoparticles of controllable size by the reduction of aqueous chloroaurate ions by hexadecylaniline at the liquid-liquid interface,” Chemical Communications, no. 13, pp. 1334–1335, 2002. View at: Google Scholar
  15. K. Okitsu, A. Yue, S. Tanabe, H. Matsumoto, and Y. Yobiko, “Formation of colloidal gold nanoparticles in an ultrasonic field: control of rate of gold(III) reduction and size of formed gold particles,” Langmuir, vol. 17, no. 25, pp. 7717–7720, 2001. View at: Publisher Site | Google Scholar
  16. Y. Sun and Y. Xia, “Shape-controlled synthesis of gold and silver nanoparticles,” Science, vol. 298, no. 5601, pp. 2176–2179, 2002. View at: Publisher Site | Google Scholar
  17. R. K. Mehra and D. R. Winge, “Metal ion resistance in fungi: molecular mechanisms and their regulated expression,” Journal of Cellular Biochemistry, vol. 45, no. 1, pp. 30–40, 1991. View at: Google Scholar
  18. P. Mukherjee, S. Senapati, D. Mandal et al., “Extracellular synthesis of gold nanoparticles by the fungus Fusarium oxysporum,” ChemBioChem, vol. 3, no. 5, pp. 461–463, 2002. View at: Publisher Site | Google Scholar
  19. A. Ahmad, P. Mukherjee, S. Senapati, M. I. Khan, R. Kumar, and M. Sastry, “Extra-intracellular biosynthesis of gold nanoparticles by an alkalotolerant fungus Trichothecium sp,” Journal of Biomedical Nanotechnology, vol. 1, no. 1, pp. 47–53, 2005. View at: Google Scholar
  20. B. D. Sawle, B. Salimath, R. Deshpande, M. D. Bedre, B. K. Prabhakar, and A. Venkataraman, “Biosynthesis and stabilization of Au and Au-Ag alloy nanoparticles by fungus, Fusarium semitectum,” Science and Technology of Advanced Materials, vol. 9, no. 3, pp. 035012–035017, 2008. View at: Publisher Site | Google Scholar
  21. S. He, Z. Guo, Y. Zhang, S. Zhang, J. Wang, and N. Gu, “Biosynthesis of gold nanoparticles using the bacteria Rhodopseudomonas capsulata,” Materials Letters, vol. 61, no. 18, pp. 3984–3987, 2007. View at: Publisher Site | Google Scholar
  22. M. I. Husseiny, M. A. El-Aziz, Y. Badr, and M. A. Mahmoud, “Biosynthesis of gold nanoparticles using Pseudomonas aeruginosa,” Spectrochimica Acta Part A, vol. 67, no. 3-4, pp. 1003–1006, 2007. View at: Publisher Site | Google Scholar
  23. K. Kalimuthu, R. S. Babu, D. Venkataraman, M. Bilal, and S. Gurunathan, “Biosynthesis of silver nanocrystals by Bacillus licheniformis,” Colloids and Surfaces B, vol. 65, no. 1, pp. 150–153, 2008. View at: Publisher Site | Google Scholar
  24. J. Y. Song, H. K. Jang, and B. S. Kim, “Biological synthesis of gold nanoparticles using Magnolia kobus and Diopyros kaki leaf extracts,” Process Biochemistry, vol. 44, no. 10, pp. 1133–1138, 2009. View at: Publisher Site | Google Scholar
  25. S. S. Shankar, A. Rai, B. Ankamwar, A. Singh, A. Ahmad, and M. Sastry, “Biological synthesis of triangular gold nanoprisms,” Nature Materials, vol. 3, no. 7, pp. 482–488, 2004. View at: Publisher Site | Google Scholar
  26. A. K. Singh, M. Talat, D. P. Singh, and O. N. Srivastava, “Biosynthesis of gold and silver nanoparticles by natural precursor clove and their functionalization with amine group,” Journal of Nanoparticle Research, vol. 12, no. 5, pp. 1667–1675, 2010. View at: Publisher Site | Google Scholar
  27. J. L. Gardea-Torresdey, J. G. Parsons, E. Gomez, J. Peralta-Videa, H. E. Troiani, and P. Santiago, “Formation and growth of Au nanoparticles inside live alfalfa plants,” Nano Letters, vol. 2, no. 4, pp. 397–401, 2002. View at: Publisher Site | Google Scholar
  28. S. P. Chandran, M. Chaudhary, R. Pasricha, A. Ahmad, and M. Sastry, “Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract,” Biotechnology Progress, vol. 22, no. 2, pp. 577–583, 2006. View at: Publisher Site | Google Scholar
  29. J. Huang, Q. Li, D. Sun et al., “Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf,” Nanotechnology, vol. 18, no. 10, p. 105104, 2007. View at: Publisher Site | Google Scholar
  30. Z. Ahmed, M. Z. Chishti, R. K. Johri, A. Bhagat, K. K. Gupta, and G. Ram, “Antihyperglycemic and antidyslipidemic activity of aqueous extract of Dioscorea bulbifera tubers,” Diabetologia Croatica, vol. 38, no. 3, pp. 63–72, 2009. View at: Google Scholar
  31. S. Ghosh, M. Ahire, S. Patil et al., “Antidiabetic activity of Gnidia glauca and Dioscorea bulbifera: potent amylase and glucosidase inhibitors,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 929051, 10 pages, 2012. View at: Publisher Site | Google Scholar
  32. T. B. Nguelefack, M. Mbiantcha, A. Kamanyi, R. B. Teponno, A. L. Tapondjou, and P. Watcho, “Analgesic and anti-inflammatory properties of extracts from the bulbils of Dioscorea bulbifera L. var sativa (Dioscoreaceae) in mice and rats,” Evidence-Based Complementary and Alternative Medicine, vol. 2011, Article ID 912935, 2011. View at: Publisher Site | Google Scholar
  33. H. Gao, M. Kuroyanagi, L. Wu, N. Kawahara, T. Yasuno, and Y. Nakamura, “Antitumor-promoting constituents from Dioscorea bulbifera L. in JB6 mouse epidermal cells,” Biological and Pharmaceutical Bulletin, vol. 25, no. 9, pp. 1241–1243, 2002. View at: Publisher Site | Google Scholar
  34. R. B. Teponno, A. L. Tapondjou, J. D. Djoukeng et al., “Isolation and NMR assignment of a pennogenin glycoside from Dioscorea bulbifera L. var sativa,” Natural Product Sciences, vol. 12, no. 1, pp. 62–66, 2006. View at: Google Scholar
  35. H. Gao, B. Hou, M. Kuroyanagi, and L. Wu, “Constituents from anti-tumor-promoting active part of Dioscorea bulbifera L. in JB6 mouse epidermal cells,” Asian Journal of Traditional Medicines, vol. 2, no. 3, pp. 104–109, 2007. View at: Google Scholar
  36. M. R. Bhandari and J. Kawabata, “Organic acid, phenolic content and antioxidant activity of wild yam (Dioscorea spp.) tubers of Nepal,” Food Chemistry, vol. 88, no. 2, pp. 163–168, 2004. View at: Publisher Site | Google Scholar
  37. M. Zayats, R. Baron, I. Popov, and I. Willner, “Biocatalytic growth of Au nanoparticles: from mechanistic aspects to biosensors design,” Nano Letters, vol. 5, no. 1, pp. 21–25, 2005. View at: Publisher Site | Google Scholar
  38. Y. Xiao, V. Pavlov, S. Levine, T. Niazov, G. Markovitch, and I. Willner, “Catalytic growth of Au nanoparticles by NAD(P)H cofactors: optical sensors for NAD(P)+-dependent biocatalyzed transformations,” Angewandte Chemie—International Edition, vol. 43, no. 34, pp. 4519–4522, 2004. View at: Publisher Site | Google Scholar
  39. I. Willner, R. Baron, and B. Willner, “Growing metal nanoparticles by enzymes,” Advanced Materials, vol. 18, no. 9, pp. 1109–1120, 2006. View at: Publisher Site | Google Scholar
  40. S. S. Shankar, S. Bhargava, and M. Sastry, “Synthesis of gold nanospheres and nanotriangles by the Turkevich approach,” Journal of Nanoscience and Nanotechnology, vol. 5, no. 10, pp. 1721–1727, 2005. View at: Publisher Site | Google Scholar
  41. A. Rai, A. Singh, A. Ahmad, and M. Sastry, “Role of halide ions and temperature on the morphology of biologically synthesized gold nanotriangles,” Langmuir, vol. 22, no. 2, pp. 736–741, 2006. View at: Publisher Site | Google Scholar
  42. R. B. Teponno, A. L. Tapondjou, D. Gatsing et al., “Bafoudiosbulbins A, and B, two anti-salmonellal clerodane diterpenoids from Dioscorea bulbifera L. var sativa,” Phytochemistry, vol. 67, no. 17, pp. 1957–1963, 2006. View at: Publisher Site | Google Scholar
  43. S. K. Satpute, I. M. Banat, P. K. Dhakephalkar, A. G. Banpurkar, and B. A. Chopade, “Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms,” Biotechnology Advances, vol. 28, no. 4, pp. 436–450, 2010. View at: Publisher Site | Google Scholar
  44. S. K. Satpute, A. G. Banpurkar, P. K. Dhakephalkar, I. M. Banat, and B. A. Chopade, “Methods for investigating biosurfactants and bioemulsifiers: a review,” Critical Reviews in Biotechnology, vol. 30, no. 2, pp. 127–144, 2010. View at: Publisher Site | Google Scholar
  45. N. R. Jana, L. Gearheart, and C. J. Murphy, “Seed-mediated growth approach for shape-controlled synthesis of spheroidal and rod-like gold nanoparticles using a surfactant template,” Advanced Materials, vol. 13, no. 18, pp. 1389–1393, 2001. View at: Publisher Site | Google Scholar
  46. B. Nikoobakht and M. A. El-Sayed, “Preparation and growth mechanism of gold nanorods (NRs) using seed-mediated growth method,” Chemistry of Materials, vol. 15, no. 10, pp. 1957–1962, 2003. View at: Publisher Site | Google Scholar
  47. N. R. Jana, L. Gearheart, and C. J. Murphy, “Wet chemical synthesis of high aspect ratio cylindrical gold nanorods,” Journal of Physical Chemistry B, vol. 105, no. 19, pp. 4065–4067, 2001. View at: Publisher Site | Google Scholar
  48. N. R. Jana, L. Gearheart, and C. J. Murphy, “Wet chemical synthesis of silver nanorods and nanowires of controllable aspect ratio,” Chemical Communications, no. 7, pp. 617–618, 2001. View at: Google Scholar
  49. M. Tréguer-Delapierre, J. Majimel, S. Mornet, E. Duguet, and S. Ravaine, “Synthesis of non-spherical gold nanoparticles,” Gold Bulletin, vol. 41, no. 2, pp. 195–207, 2008. View at: Google Scholar
  50. Y. Wang, X. He, K. Wang, X. Zhang, and W. Tan, “Barbated Skullcup herb extract-mediated biosynthesis of gold nanoparticles and its primary application in electrochemistry,” Colloids and Surfaces B, vol. 73, no. 1, pp. 75–79, 2009. View at: Publisher Site | Google Scholar
  51. B. D. Cullity and S. R. Stock, Elements of X-Ray Diffraction, Prentice Hall, Upper Saddle River, NJ, USA, 3rd edition, 2001.
  52. R. B. Teponno, A. L. Tapondjou, E. Abou-Mansour, H. Stoeckli-Evans, P. Tane, and L. Barboni, “Bafoudiosbulbins F and G, further clerodane diterpenoids from Dioscorea bulbifera L. var sativa and revised structure of Bafoudiosbulbin B,” Phytochemistry, vol. 69, no. 12, pp. 2374–2379, 2008. View at: Publisher Site | Google Scholar
  53. M. Wij and S. Rangaswami, “Chemical constituents of Dioscorea bulbifera isolation and structure of a new dihydro phenanthrene 2,4,6,7, tetrahydroxy -9,10 dihydro phenanthrene and a new phenanthrene 2,4,5,6 tetra hydroxy phenanthrene,” Indian Journal of Chemistry Section B, vol. 16, no. 7, pp. 643–644, 1978. View at: Google Scholar
  54. M. R. Bhandari, T. Kasai, and J. Kawabata, “Nutritional evaluation of wild yam (Dioscorea spp.) tubers of Nepal,” Food Chemistry, vol. 82, no. 4, pp. 619–623, 2003. View at: Publisher Site | Google Scholar
  55. M. R. Bhandari and J. Kawabata, “Assessment of antinutritional factors and bioavailability of calcium and zinc in wild yam (Dioscorea spp.) tubers of Nepal,” Food Chemistry, vol. 85, no. 2, pp. 281–287, 2004. View at: Publisher Site | Google Scholar
  56. A. R. Shahverdi, E. A. D. Haratifar, H. R. Shahverdi et al., “Semi-biosynthesis of magnetite-gold composite nanoparticles using an ethanol extract of Eucalyptus camaldulensis and study of the surface chemistry,” Journal of Nanomaterials, vol. 2009, Article ID 962021, 2009. View at: Publisher Site | Google Scholar
  57. T. Komori, “Glycosides from Dioscorea bulbifera,” Toxicon, vol. 35, no. 10, pp. 1531–1535, 1997. View at: Publisher Site | Google Scholar
  58. G. Socrates, Infrared and Raman Characteristic Group Frequencies, John Wiley & sons, 3rd edition, 2001.
  59. B. Ankamwar, “Biosynthesis of gold nanoparticles using leaf extract,” E-Journal of Chemistry, vol. 7, no. 4, pp. 1334–1339, 2010. View at: Google Scholar
  60. S. Chen and K. Kimura, “Synthesis and characterization of carboxylate-modified gold nanoparticle powders dispersible in water,” Langmuir, vol. 15, no. 4, pp. 1075–1082, 1999. View at: Google Scholar

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