Preparation and Characterization of Water-Soluble Chitosan Microparticles Loaded with Insulin Using the Polyelectrolyte Complexation Method

Table 3

The optimal conditions for the formulation of insulin-loaded WSC-MPs by orthogonal design (L₉ (3⁴)) (n = 3).

NO.	pH of  WSC	𝐶WSC (mg/mL)	𝐶INS (mg/mL)	𝐶TPP (mg/mL)	AE(%)
1	3.8	1.0	0.5	0.7	32.01
2	3.8	1.5	0.7	1.0	27.27
3	3.8	2.0	1.0	1.5	26.32
4	4.5	1.0	0.7	1.5	21.79
5	4.5	1.5	1.0	0.7	31.24
6	4.5	2.0	0.5	1.0	44.56
7	5.0	1.0	1.0	1.0	34.91
8	5.0	1.5	0.5	1.5	27.65
9	5.0	2.0	0.7	0.7	15.95