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Journal of Nanomaterials
Volume 2011, Article ID 436528, 6 pages
Research Article

Preparation of Nanoporous Polymer Films for Real-Time Viability Monitoring of Cells

1Division of Pediatric Surgery, Department of Surgery, Taichung Veterans General Hospital, 160 Section 3 Chung-Kang Road, Taichung 40705, Taiwan
2Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, 1 University Road Puli, Nantou 54561, Taiwan
3Department of Applied Chemistry, National Chi Nan University, 1 University Road Puli, Nantou 54561, Taiwan
4Department of Applied Materials and Optoelectronic Engineering, National Chi Nan University, 1 University Road, Puli, Nantou 54561, Taiwan

Received 19 May 2010; Accepted 1 July 2010

Academic Editor: Lu Sun

Copyright © 2011 Chia-Man Chou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We have demonstrated an alternative way to monitor the viability of cells adhered on a nanoporous polymer film in real time. The nanoporous polymer films were prepared by laser interference pattering. During exposure of holographic patterning, the dissolved solvents were phase separated with photocured polymer and the nanopores were created as the solvents evaporated. The diffracted spectra from the nanoporous polymer film responded to each activity of the cell cycle, from initial cell seeding, through growth, and eventual cell death. This cell-based biosensor uses a nanoporous polymer film to noninvasively monitor cell viability and may prove useful for biotechnological applications.