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Journal of Nanomaterials
Volume 2011 (2011), Article ID 721631, 8 pages
Research Article

Antimicrobial Bacterial Cellulose-Silver Nanoparticles Composite Membranes

1Institute of Chemistry, São Paulo State University (UNESP), 14801-970 Araraquara, SP, Brazil
2Instituto de Ciência e Tecnologia (ICT), Universidade Federal de Alfenas (UNIFAL-MG), Campus Avançado de Poços de Caldas, Rua Gabriel Monteiro da Silva, 37130-000 Alfenas, MG, Brazil
3Uniara, 14801-320 Araraquara, SP, Brazil

Received 13 December 2010; Accepted 21 February 2011

Academic Editor: Edward Andrew Payzant

Copyright © 2011 Hernane S. Barud et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Antimicrobial bacterial cellulose-silver nanoparticles composite membranes have been obtained by “in situ” preparation of Ag nanoparticles from hydrolytic decomposition of silver nitrate solution using triethanolamine as reducing and complexing agent. The formation of silver nanoparticles was evidenced by the X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and absorption in the UV-Visible (350 nm to 600 nm). Thermal and mechanical properties together with swelling behavior for water were considered. TEA concentration was observed to be important in order to obtain only Ag particles and not a mixture of silver oxides. It was also observed to control particle size and amount of silver contents in bacterial cellulose. The composite membranes exhibited strong antimicrobial activity against Gram-negative and Gram-positive bacteria.

1. Introduction

Bacterial cellulose (BC) is a nanomaterial produced by various strains of Acetobacter species and also strains of pseudomonas, Achrobacter, Alcaligene, Aerobacter, and Azotobacter [1]. Acetobacter xylinum (or Gluconacetobacter xylinus) is one of the species capable to produce cellulose in large scale in culture medium containing carbon and nitrogen sources in either static or agitated environment.

BC is formed by a network of ribbon-shaped cellulose fibrils that are less than 100 nm wide and made of microfibrils 2–4 nm in diameter [2].

In terms of the molecular formula, BC is identical to cellulose of plant origin—apart from alien groups such as carbonyl and carboxyl units in the latter as a result of the plant cellulose processing. However, important structural features and properties, significant for practical application of BC, are quite different from wood cellulose: high purity, high degree of polymerization (up to 8000), high crystallinity (of 70 to 80%), high water content to 99%, and high mechanical stability [3].

As a consequence of the special properties, a diversity of applications are proposed as industry of paper and textiles, diaphragms for electroacoustic transducers, paint additives, coatings, pharmaceuticals and cosmetics, optically transparent composites, substrates for OLEDs, e-paper and as biomaterials in cosmetics and medicine [35].

In fact, one of the most promising areas of BC application as biomaterials is the utilization as a temporary skin substitute in the therapy of difficult wounds, burns, and ulcers. However, BC itself has no antimicrobial activity to prevent wound infection [4].

In this sense, recently, a great effort has been devoted to the development of antimicrobial BC membranes especially silver particles containing BC [68].

Silver has been known to exhibit strong cytotoxicity towards a broad range of micro-organisms, based on oligodynamic effect, that is, silver ions are capable of causing a bacteriostatic (growth inhibition) or even a bactericidal (antibacterial) impact [9].

In a previous work, “in situ” preparation of silver nanoparticle containing BC membranes was shown with the hydrolytic decomposition of silver nitrate using triethanolamine (TEA) complexes. The utilization of TEA as stabilizer and reducing agent leads to spherical particles well dispersed on the BC bulk ultrafine reticulated structure. Therefore, a mixture of Ag and Ag oxides was, in fact, obtained due to low TEA content [10].

In this work, a systematic study involving the hydrolytic decomposition of silver nitrate solution at different triethanolamine (TEA) concentrations is presented. Antimicrobial tests for the BC-silver nanoparticles composite membranes were also considered.

2. Material and Methods

2.1. Bacterial Cellulose Membranes

Never dried bacterial cellulose (4 mm thick) membranes were supplied from Fibrocel-Produtos Biotecnológicos LTDA-Brazil.

2.2. BC-Silver Nanoparticles Composites Preparation

Bacterial cellulose membranes (6 cm2) were soaked in 15 mL of 0.01 mol·L−1 AgNO3 solution, followed by the addition of 0.1 mL of TEA solutions with different concentrations (0.01 mol·L-1, 0.1 mol·L-1 and 1.0 mol·L-1). The mixtures were kept for 12 hours. After that, samples were washed several times in 30% ethanol solution. Silver-containing BC membrane membranes were dried at 80°C for 24 hours.

2.3. Characterization
2.3.1. X-Ray Diffraction (XDR)

XRD patterns were obtained in a Siemens Kristalloflex diffractometer using nickel-filtered Cu Kα radiation, step pass of 0.02° and a step time of 3 s, from 4 to 70° (2θ angle).

2.3.2. Scanning Electron Microscopy

Scanning electron microscope images were obtained with an FEG-SEM JSM 6330F at the LNLS (Brazilian Synchroton Light Laboratory). Samples were coated with a thick carbon layer.

2.3.3. Transmission Electron Microscopy (TEM)

TEM measurements were obtained in a JEOL 200CX microscopy, operating at 200 kV. The samples were heated at 600°C degrees to remove bacterial cellulose. The residue formed by silver nanoparticles was suspended in ethanol. A drop of the suspension was deposited on the copper grid. The information was added in the text. A drop of the suspension was deposited on the copper grid.

2.3.4. Thermogravimetric (TG) Curves

Thermal gravimetric (TG) curves were obtained for dried samples in an SDT 2960 equipment from TA-Instruments. Samples were heated in open alumina pans from 40°C to 600°C, under synthetic air with flux of 50 mL min−1, at heating rate of 10°C/min.

2.3.5. UV-VIS

UV-V absorption spectra were obtained in a Varian Cary 500 Scan spectrometer. Pure and dry cellulose membranes were used as references in an appropriate support for solid samples.

2.3.6. Swelling

BC and BC-Ag-TEA membranes, dried to constant weight, were cut into disc shapes with 1.5 cm diameter and immersed in deionized water for different times at room temperature. Swelling was calculated as follows: where is the initial weight of dried sample and is the weight of sample in swollen state.

2.3.7. Mechanical Properties

The mechanical measurements were performed with a dynamic mechanical analyzer (DMA) 2980 (TA Instruments) equipped with a film tension clamp at 27°C. The specimens dimensions were 30.50 mm × 6.20 mm × 0.15 mm (length × width × thickness). A preload force of 0.01 N was used and the force ramp 8 N m-1 until the rupture of sample. The device was previously calibrated, and a total of 5 measurements for each sample were made in order to ensure the reproducibility of the results.

2.3.8. Antimicrobial Activity Studies

Antimicrobial activities have been investigated against Pseudomonas aeruginosa ATCC-27853 and Escherichia coli ATCC 25922 as models for Gram-negative bacteria and Staphylococcus aureus ATCC 25923 as model for Gram-positive bacteria. The antimicrobial activity was evaluated by the disc diffusion method in Mueller-Hinton (MH) agar plates. The microorganisms were cultivated in tubes containing 3 mL of sterile brain heart infusion (BHI) and incubated for 18 h at 35°C –37°C. Sufficient inoculum was added in new tubes until turbidity of 0.5 McFarland (~108 CFU/mL). The new bacterial inoculum was uniformly spread using sterile cotton swabs on sterile Petri dishes MH agar. Sample membranes were cut into disc shapes of 1.5 cm diameter and UV sterilized for 2 hours. After sterilization, the discs were placed on the bacterial culture, and the plates were incubated for 18 h at 35°C –37°C. Clear zones of inhibition formed around the discs were measured.

3. Results and Discussion

3.1. X-Ray Diffraction (XRD)

Figure 1 shows XRD patterns of BC-Ag-TEA membranes.

Figure 1: X-rays diffraction patterns of BC-Ag-TEA membranes. (a) BC-TEA 0.01 mol·L-1, (b) BC-TEA 0.1 mol·L-1, and (c) BC-TEA 1.0 mol·L-1.

Figure 1(a) shows the XRD pattern obtained for the sample with the smallest concentration of TEA. A diffraction band is observed at 32.3° and can be attributed to AgO or Ag2O phases. The same behavior was observed for the sample BC-Ag-TEA 0.1 mol·L-1 (Figure 1(b)) with a small decrease in the relative intensity of the line assigned to the oxides mixture.

Figure 1(c) shows the XRD pattern obtained for the sample BC-Ag-TEA 1 mol·L-1. The two diffraction bands at 38.3° and 44.5° can be attributed to metallic silver corresponding to the crystallographic planes (111) and (220), respectively [6, 10]. The band corresponding to the oxide mixture is not observed, confirming, therefore, the influence of the concentration of TEA in obtaining metallic silver.

As discussed in our preview paper [10], the formation of Ag nanoparticles on BC fibers can be explained in terms of the interaction with cellulose hydroxyl groups. Despite of the fact that cellulose structure exhibits aldehyde hydrates reducing terminal groups, no metallic silver peak could be observed in XRD patterns of membranes prepared only from BC and Ag+ solutions. Diffusion of hydrated silver ions [Ag(H2O)2]+ into bacterial cellulose matrix leads to coordination with the different cellulose hydroxyl groups. In general, the reducing action of different agents (UV radiation, sodium boron hydride, hypophosphite, hydrazine, hydroxylamine, ascorbic acid, among others) is required [1114].

In this work, the additional interaction with TEA should lead to a more complex reduction mechanism. TEA molecules act simultaneously as a strong reducing agent and chelating agent, influencing the nucleation and particle growth, hence particle size. Hydrolytic decomposition of Ag-TEA complexes in aqueous solutions at around 50°C is known to lead to Ag and AgO thin films [15]. TEA acts as a tridentate ligand through two of the three hydroxyl O atoms together with the amine N atom. Ag+ is reduced to Ag(0)2, and as these particles are formed, they act as a catalyst for the reduction of the remaining metal ions present in the bulk solution leading to Ag(0)n cluster growth. The aggregation process does not cease until all metal ions in solutions are consumed, resulting in larger particles [15]. The hydrolytic decomposition of the Ag-TEA complexes leads to silver particles formation stabilized on the BC fibrils. Moreover, depending on the Ag+ and TEA relative contents, Ag+ ions could be sandwiched by two TEA molecules. Intra- and intermolecular hydrogen bonding among hydroxyl TEA groups could lead to a three-dimensional network and the decomposition of this three-dimensional complex network allows precipitation of spherical particles on BC surface.

The observation of AgO phase in XRD diffraction pattern corroborates the decomposition mechanism for Ag-TEA complexes proposed in [15], since the low TEA/silver concentration should lead to a Ag(0)-AgO mixture. In fact, XRD analyses confirms that TEA concentration is very important in order obtain only Ag particles, as observed in the BC-Ag-TEA 1 mol·L-1 diffraction pattern [15].

3.2. SEM and TEM Images

Figure 2 shows SEM images of pure bacterial cellulose and a representative image for BC-Ag-TEA composite.

Figure 2: SEM images of: (a) Pure BC membrane and (b) BC-Ag-TEA 1 mol·L-1.

The dried BC membrane, Figure 2(a) shows a 3D network structure composed by long cellulose nanofibers.

BC-Ag-TEA 1 M membrane, Figure 2(b) shows well dispersed spherical silver particles (white spots) attached to the BC membrane and adsorbed on the fibrils surface, and this image suggest that BC fibrils appear swollen most probably by TEA presence.

Figure 3 shows TEM images and histograms obtained for BC-Ag-TEA samples. Spherical nanoparticles were obtained for all TEA concentrations. Bigger particles are obtained with increasing TEA content. In fact, smaller mean particle size (8 nm) is observed for BC-Ag-TEA 0.01 mol·L-1 membranes while particles with mean size of 15 nm are observed for BC-Ag-TEA 1 mol·L-1 membrane. According to Rira Jung, the increase in particles size observed with the increase in the TEA content phenomenon should occur, because the reduction rate observed for the higher TEA concentration is relatively higher than the adsorption rate of the formed silver nanoparticles on cellulose nanofibrils. Therefore, the newly reduced silver nanoparticles might form aggregates with silver crystals that had already been adsorbed on the cellulose nanofibrils [7].

Figure 3: TEM images and respective histograms: (a) BC-Ag-TEA 0.01 mol·L-1; (b) BC-Ag-TEA 0.1 mol·L-1; (c) BC-Ag-TEA 1 mol·L-1.
3.3. Thermal Gravimetric (TG) Analyses

Figure 4 shows the thermal behavior of the BC and BC-Ag-TEA membranes.

Figure 4: TG curves for: (a) BC; (b) BC-Ag-TEA 0.01 mol·L-1; (c) BC-Ag-TEA 0.1 mol·L-1; (d) BC-Ag-TEA 1 mol·L-1. The inset shows the Ag content (%) in the membranes as a function of TEA concentration used in the preparation procedure.

A continuous mass loss (5–10%) is observed for pure BC, in the temperature range between 50°C until 150°C, due to evaporation of the superficial water. Thermal degradation occurs in two steps at approximately 330°C and 460°C [2].

The BC-Ag-TEA 0.01 mol·L-1 membrane presents similar behavior. The low Ag-TEA content does not lead to important changes in TG curve.

A different thermal profile is observed for BC-Ag membranes containing higher concentration of TEA complex. The mass loss due to water evaporation is no longer observed for temperatures up to 110°C. New mass loss events (around 30%) are observed up to 250 degrees for BC-Ag-TEA 0.1 mol·L-1 and BC-Ag-TEA 1 mol·L-1 that must be associated to water evaporation and TEA evaporation and degradations [16].

From TG curves carried out in static air, the residual mass could be used to estimate the silver contents into BC membrane. The inset in Figure 5 shows increasing silver particles content with the increase in TEA concentrations. The results show clearly the important role of TEA in the silver adsorption process.

Figure 5: Absorption spectra of silver nanoparticle bacterial cellulose membranes. (a) BC-Ag-TEA 0.01 mol·L-1; (b) BC-Ag- TEA 0.1 mol·L-1; (c) BC-Ag-TEA 1 mol·L-1.
3.4. UV-VIS

Figure 5 shows absorption spectra.

The color of BC-Ag-TEA composites changed from yellow-brown to black with increase on TEA concentration and consequently due to the increases on silver content [17, 18].

The typical absorption of metallic silver nanoparticles was observed for all samples, related to the surface plasmon resonance (SPR) of conducting electron (or free electron) on the surface of silver nanoparticles. Maxima are observed at 427 nm for BC-Ag-TEA 0.01 mol·L-1 and BC-Ag-TEA 0.1 mol·L-1 membranes and at 452 nm for the BC-Ag-TEA 1 mol·L-1 sample. Broadening and red shift of the absorption band observed for the membrane obtained with the higher TEA content may be associated with the increase in a particle size and size distribution [6, 17, 18].

3.5. Swelling

Figure 6a) and (b) show swelling (deionized water) observed for BC and CB-Ag-TEA 1 mol·L-1 membranes.

Figure 6: Swelling ability of (a) BC pure membrane; (b) BC-Ag-TEA 1 mol·L-1 membrane.

The main absorption occurs in the first two hours, and maximum swelling of 11 is observed for BC membrane, Figure 6(a). This behavior must be due to both chemical and physical structure of bacterial cellulose. BC is hydrophilic and it absorb a large amount of water; concerning the physical structure, bacterial cellulose is 3D nonwoven network with large amount of pores generating the capillary forces contributing to water sorption [3].

Figure 6(b) shows the diminishing on the swelling ability (maximum swelling of 7) observed for the BC-Ag-TEA 1 mol·L-1 sample. The decrease in the capacity of absorption must refer to the reduction of the amounts of BC pores due to the incorporation of the silver nanoparticles and also to the plasticizer role observed for TEA containing samples which leads do reduction is swelling behavior. Swelling may also have been decreased due to unavailable hydrophilic groups (hydroxyl/aldehyde) which had reduced the silver and are no longer available to bind water. This plasticizer role of TEA is more clearly observed on mechanical properties shown hereafter.

3.6. Mechanical Properties

Figure 7 shows results for stress-strain measurements. Table 1 summarizes the derived results.

Table 1: Tensile modulus, tensile strength, and elongation at break of BC membrane and BC-Ag-TEA composites.
Figure 7: Stress × strain curves: (a) Pure BC membrane; (b) BC-Ag-TEA 0.01 mol·L-1; (c) BC-Ag-TEA 0.1 mol·L-1; (d) BC-Ag-TEA 1 mol·L-1.

Pure BC membranes display good mechanical properties. High toughness is observed for BC-derived materials and composites due mainly to the nanofibrilar network structure and high mechanical nanofibril performance [19].

In fact, values of tensile modulus for single filaments of bacterial cellulose have been evaluated through AFM and Raman measurements and values of 78 GPa and 114 GPa have been obtained, respectively [20, 21].

The curve obtained for the pure BC membrane, as shown in Figure 7(a), displays an initial linear behavior followed by a plastic behavior. The tensile modulus value obtained from the linear part of the curve is of 11 GPa in accordance with results from the literature [22].

The observation of a plastic region is related to the breakdown within the fibers network during deformation and the rupture (tensile strenght) occurs at 98 MPa with 1.2% elongation.

The addition of TEA lowers tensile modulus down to the value of 1.2 GPa observed for the BC-Ag-TEA 1 mol·L-1 as shown in Table 1. Figure 8 also shows the plasticizer role played by TEA already observed for other polymer [23, 24].

Figure 8: Antimicrobial activity of BC-Ag-TEA 1 M: (a) Staphylococcus aureus; (b) Pseudomonas aeruginosa.

More pronounced values are observed for the elongation at break as TEA content increases. For the samples studied, here the values are almost doubled with the addition of TEA. Tensile strength is observed to decrease. Comparing the samples containing TEA by increasing TEA content tensile strength is observed to increase.

3.7. Antimicrobial Activity Studies

Antimicrobial tests for all BC-Ag-TEA composites show strong antimicrobial activity against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria.

Figure 7 shows only a representative result for antimicrobial activity of the BC-Ag-TEA 1 mol·L-1 membrane toward Gram-positive and Gram-negative Staphylococcus aureus and Pseudomonas aeruginosa, respectively. An inhibition zone of 2 cm was observed confirming the diffusion of silver nanopartiles from BC-Ag-TEA composite to culture medium [18]. No inhibition zone was observed for the pure bacterial cellulose control.

The mechanism for microbial growth inhibition observed for silver is not entirely understood. Possible mechanisms involve the interaction of silver ions with biological macromolecules through proteins thiol groups (-SH). Monovalent silver ions (Ag+) would replace (H+) ions of sulfhydryl or thiol groups, inactivating the protein, decreasing membrane permeability, and eventually causing cellular death. The reaction of monovalent silver with sulfhydryl groups produces a much more stable -S-Ag group only on the bacterial cell surface. Silver ions may not be capable of permeating through cell membranes to react with the interior -S-H groups, rendering silver relatively nontoxic to humans and animals [25].

4. Conclusion

Antimicrobial BC-silver nanoparticles composite membranes were prepared using TEA as complexing and reducing agent and also plasticizer.

TEA molecules play a multifunctional role acting simultaneously as a strong reducing agent and good silver ions chelating agent, influencing the nucleation and particle growth, and hence particle size. TEA concentration is determinate in order to obtain only Ag particles and not a mixture of silver oxides, and TEA concentration was also observed to control particle size and amount of silver contents on to bacterial cellulose.

TEA also plays the role of plasticizer for the membranes as suggested from mechanical properties results. The new composite membranes exhibited a strong antimicrobial activity against Staphylococcus aureus (Gram-positive bacteria), Pseudomonas aeruginosa and Escherichia coli (Gram-negative bacteria), which are bacteria commonly found in contaminated wounds.


The authors acknowledge the financial support of the FAPESP, CAPES, and CNPq. They also thank LME/LNLS for technical support during electron microscopy work and the company Fibrocel-Produtos Biotecnológicos Ltda. for supplying the bacterial cellulose membranes.


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