Design and Construction of a One-Dimensional DNA Track for an Artificial Molecular Motor

Figure 5

Agarose gel electrophoresis of BbsI/BsaI double-digested plasmids containing repeat sequences (ABC)_𝑁. Lanes 1 to 6 correspond to 𝑁 = 1, 2, 4, 8, 16, and 32. Lane 7 shows an unexpected 𝑁=24 clone generated from a doubling of pK₁₆. Lanes 8 and 9 are controls (digest of the parent pYIC plasmid and 1 kbp ladder, resp.). Numbers on the right indicate fragment lengths in base pairs.