Nanopolymers Delivery of the Bone Morphogenetic Protein-4 Plasmid to Mesenchymal Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo
Figure 1
MSCs surface markers (a), cytotoxicity (b), and transfection efficiency (c) of PLGA nanopolymers. The mesenchymal-related antigens CD44 and CD90 were positive, but the hematopoietic-related antigens CD14 and CD45 were negative (a). The cell viability of the experimental cells (MSCs being transfected with BMP-4 plasmid by PLGA nanopolymers) significantly, decreased than that of the control cells (MSCs being transfected with naked BMP-4 plasmid alone) 72 hours after transfection. The cell viability was decreased with the N/D ratio increasing (b). The transfection efficiency of PLGA nanopolymers significantly increased than that of naked BMP-4 plasmid alone 72 hours after transfection, and was increased with the N/D ratio increasing (c). *, **.
