Table of Contents Author Guidelines Submit a Manuscript
Journal of Nanomaterials
Volume 2012 (2012), Article ID 398720, 7 pages
Research Article

Enhanced Oxidative Stress and Physiological Damage in Daphnia magna by Copper in the Presence of Nano-TiO2

Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, Beihang University, Beijing 100191, China

Received 9 May 2012; Accepted 23 May 2012

Academic Editor: Jiaguo Yu

Copyright © 2012 W. H. Fan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study examines the potential hazard of an individual nanomaterial on the Cu biotoxicity to aquatic organisms. Daphnia magna in the absence or presence of nano-TiO2 was exposed to Cu. Maintaining nano-TiO2 at a safe concentration cannot eliminate its potential hazard. The biomarkers superoxide dismutase, catalase, and Na+/K+-ATPase in D. magna were measured. Cu in the presence of nano-TiO2 induced higher levels of oxidative stress and physiological damage because of the sorption of Cu. Nano-TiO2 also caused Na+/K+-ATPase inhibition possibly by impeding the Na+/K+ transfer channel. The correlations among the biomarkers, mortality, and accumulation further showed that the overloading reactive oxygen species generation caused by nano-TiO2 contributed to deeper oxidative stress and physiological regulation, thereby causing greater toxic injury.